System Description; Sample Chamber Atmosphere; Contracon Decontamination Routine - Kendro HERAcell 240 Operating Instructions Manual

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HERAcell 240 CO Incubator Operating Instructions
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5.

System Description

5.1

Sample Chamber Atmosphere

The special physiological conditions for the preparati-
on and cultivation of cell and tissue cultures are si-
mulated in the incubator's sample chamber. In this,
the sample chamber atmosphere is determined by
three factors:
•
Temperature;
•
Relative humidity;
•
CO concentration.
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Temperature:
For fault-free operation, the ambient temperature in
the operating area should be at least 18 °C, while the
incubation temperature should be at least 3 °C above
the ambient room temperature.
The heating system regulates the incubation tempe-
rature from the minimum of 5 °C above ambient tem-
perature up to 55 °C. The air jacket heating principle
together with the separate exterior door heater ensure
that condensation will not form on the sample cham-
ber's side walls and ceiling.
Relative Humidity:
The floor pan in the sample chamber can hold 4.5 l of
treated water. The sample chamber heater promotes
the evaporation of this water thus providing a constant
humidity level in the sample chamber. Under normal
operating conditions at a standard incubation tempe-
rature of 37 °C, a constant relative humidity of approx.
95 % develops in the sample chamber
A high/low switching function allows the unit to be
switched between two modes to regulate the humidity:
•
Setting 0 (High) creates a relative humidity of
approx. 95 % in the sample chamber (standard
setting),
•
Setting I (Low) a relative humidity of approx. 90
% is created.
Version: 03.2001 / 50065640
Only treated water which meets both of the following
criteria is required for humidifying:
CO
CO
growth conditions for the cell and tissue cultures.
The pH value of the bicarbonate-buffered culture me-
dium is significantly influenced by the CO
the sample chamber atmosphere.
The CO
can be regulated through a range of 0 - 20 %.
The supplied CO
properties:
The ContraCon decontamination routine decontami-
nates the entire sample chamber, together with all in-
stalled components and sensors.
.
During this routine, a hot/moist atmosphere is main-
tained at 90 °C providing a high decontamination ef-
fect.
The effectiveness of the ContraCon decontamination
routine has been documented by independent institu-
tions. Kendro can provide information concerning the-
se tests upon request.
The ContraCon decontamination routine requires ap-
prox. 25 hours to run a complete cycle.
Once the routine has concluded, the unit must be re-
started with the auto-start routine.
- 21 -
•
Distilled;
•
Autoclaved, sterile
Supply:
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is supplied to the sample chamber to ensure the
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content of the sample chamber atmosphere
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must exhibit one of the following
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•
Minimum purity: 99.5 %;
•
Medical gas quality.
5.2

ContraCon Decontamination Routine

content of
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