Summary of Contents for Kendro HERAcell 240
- Page 1 Operating Instructions ® HERAcell 240 CO Incubator with Decontamination Routine Version: 03.2001 / 50065640...
- Page 2 ® HERAcell 240 CO Incubator Operating Instructions - 2 - Version: 03.2001 / 50065640...
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Page 3: Table Of Contents
® HERAcell 240 CO Incubator Operating Instructions Contents 1. Information regarding Safe Operation....................... 6 1.1 Operator Information............................. 6 1.2 User Information ............................7 1.3 Explanation of Symbols ..........................8 1.4 General Safety Information ......................... 10 1.5 Safety Information Concerning CO ...................... - Page 4 ® HERAcell 240 CO Incubator Operating Instructions Contents 10. Cleaning and Disinfection..........................47 10.1 Preparation ..............................47 10.2 Cleaning ..............................49 10.3 Manual Disinfection............................. 49 10.4 ContraCon Decontamination Routine......................51 10.5 ContraCon Decontamination Routine Activation ..................53 10.6 Interrupting the ContraCon Decontamination Routine................53 11.
- Page 5 ® HERAcell 240 CO Incubator Operating Instructions Index of Figures Fig. 1 Unit dimensions and minimum distances ....................16 Fig. 2 Lift points ..............................17 Fig. 3 Stacking ..............................17 Fig. 4 Drawer installation ............................. 18 Fig. 5 Front view ..............................19 Fig.
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Page 6: Information Regarding Safe Operation
The HERAcell 240 CO Incubator has been manu- For your own safety, please contact Kendro Laborato- factured according to the highest standards and is ry Products or your dealer should any questions arise operationally safe. Nonetheless, this unit presents a... -
Page 7: User Information
These operating instructions are copyrighted. Reprin- ting, photomechanical or digital processing or repro- duction – including extracts – are therefore only permitted with the written approval of Kendro Labora- tory Products GmbH. This does not apply to the reproduction for use solely within the operator’s company. -
Page 8: Explanation Of Symbols
® HERAcell 240 CO Incubator Operating Instructions Information regarding Safe Operation Explanation of Symbols Symbols Used in These Operating Instructions: Symbols in the Short Instructions: WARNING! Operator steps to be carried out on Failure to observe this instruction can result in severe the unit or fatal injury. - Page 9 ® HERAcell 240 CO Incubator Operating Instructions Information regarding Safe Operation Symbols in the Short Instructions: Symbols in the Short Instructions: Check humidifying system water Close unit doors. level and top up as required. Max. fill volume, 4.5 l; min. volume, 1.8 l Fill 350 ml water reservoir for Con- Remove samples and water reser- traCon disinfection routine...
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Page 10: General Safety Information
® HERAcell 240 CO Incubator Operating Instructions Information regarding Safe Operation General Safety Information Proper Use: Improper Use: ® The HERAcell 240 CO Incubator is a laboratory unit Cell and tissue cultures which do not meet the requi- used for the cultivation of cell and tissue cultures. rements of safety levels L1, L2, and L3 may not be The unit permits the simulation of the special physio- used in the unit. - Page 11 NOTE –Recycling-Service: • An interlock switch in the door interrupts the supply and the heater when the glass Kendro offers a proper recycling service for door is opened. old equipment. For further information regarding this • An independent overtemperature protection fee-based service, please contact the Kendro Service.
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Page 12: Safety Information Concerning Co
® HERAcell 240 CO Incubator Operating Instructions Information regarding Safe Operation Safety Information Concerning CO CAUTION – Suffocation Hazard! is classified as a gas representing a potential health hazard. Special safety measures must therefo- re be observed when starting and using the CO Incu- The release of larger amounts of CO into... -
Page 13: Initial Inspection
Packing material Should damage be discovered or if components are • Recycled paper missing, immediately inform the carrier, Kendro Labo- • Plastic foam Styrofoam (CFC free) ratory Products, or your dealer. •... -
Page 14: Parts Supplied
® HERAcell 240 CO Incubator Operating Instructions Initial Inspection Parts supplied Incubator with full glass door Incubator with 6-port gas diaphragm Equipment level Full glass door with Full glass door with di- 6-port gas diaphragm 6-port gas diaphragm continuous inserts vided inserts with divided inserts with continuous inserts... -
Page 15: Location Requirements
® HERAcell 240 CO Incubator Operating Instructions Location Requirements Ambient Conditions Room Ventilation The unit may only be operated at setup locations When CO is fed to the unit, there is a slight over- which conform to the special ambient conditions listed pressure in the sample chamber. -
Page 16: Space Requirements
Kendro offers various stands as part of its line of ac- cessory equipment (Refer to Chapter 12, “Spare Parts and Accessories” for the relevant order number). -
Page 17: Moving
® HERAcell 240 CO Incubator Operating Instructions Location Requirements Moving Figure 2 / 3 NOTE – Lift Points: Fig. 2, Lift points When moving the unit, do not lift it by its doors or other installed components such as the circuit cabi- net on the rear. -
Page 18: Door Modifications
NOTE – Modifications: Door modifications may only be performed by the Kendro Laboratory Products Service. Drawer Once the unit has been set up, the drawer is inserted between the two guide brackets under the unit body. -
Page 19: Parts Identification
® HERAcell 240 CO Incubator Operating Instructions Parts Identification Front View Figure 5 Stacking elements [11] Nameplate Cover flaps [12] Main power switch Measuring cell with fan wheel and sensors [13] Support profile Door interlock [14] Insert tray Glass door [15] Door lock, glass door Measurement opening... -
Page 20: Rear View
® HERAcell 240 CO Incubator Operating Instructions Parts Identification Rear View Figure 6 Circuit cabinet with supply connections: Fig. 6, Rear view • Gas connection • RS 232 interface • Zero-potential alarm contact • Mains power connection Access port, 42 mm dia. Pressure compensation opening - 20 - Version: 03.2001 / 50065640... -
Page 21: System Description
• Setting 0 (High) creates a relative humidity of routine has been documented by independent institu- approx. 95 % in the sample chamber (standard tions. Kendro can provide information concerning the- setting), se tests upon request. • Setting I (Low) a relative humidity of approx. 90 The ContraCon decontamination routine requires ap- % is created. -
Page 22: Sensors
® HERAcell 240 CO Incubator Operating Instructions System Description Sensors Figure 7 / 8 The fan wheel and two sensor modules are installed Fig. 7, Metering cell sensors in the base plate [2] of the metering cell: • Sensor module [1] measures the sample chamber temperature and provides overtemperature pro- tection;... -
Page 23: Supply Connections
® HERAcell 240 CO Incubator Operating Instructions System Description Supply Connections Figure 9 All supply connections are mounted on the circuit ca- Fig. 9, Supply connections binet on the rear of the unit. Gas Connection: The gas supply between the unit and the gas supply system is established using the connecting hose pro- vided. -
Page 24: Sample Chamber Layout
® HERAcell 240 CO Incubator Operating Instructions System Description Sample Chamber Layout Figure 10 The CO Incubator’s sample chamber has been de- Fig. 10, Sample chamber layout signed to have a minimal surface area to minimise contamination and to allow efficient cleaning. Interior Chamber: All sample chamber components are made of stain- less steel, having highly polished, totally smooth,... -
Page 25: Fig. 11 Water Reservoir
® HERAcell 240 CO Incubator Operating Instructions System Description Figure 11 / 12 Water Reservoir, Fig. 11: Fig. 11, Water reservoir The water reservoir [1] is integrated into the base of the inner chamber and slopes towards the rear unit wall. -
Page 26: Initial Setup
® HERAcell 240 CO Incubator Operating Instructions Initial Setup 6.1 Sample Chamber Preparation Figure 13 The CO incubator is not sterile at delivery. The unit Fig. 13, Support profile installation/removal must be cleaned before beginning operation. This applies to the following sample chamber compo- nents: •... -
Page 27: Fig. 14 Support Bracket / Insert Tray Installation
® HERAcell 240 CO Incubator Operating Instructions Initial Setup Figure 14 Support Bracket Installation: Fig.. 14, Support bracket / insert tray installation 1. Insert the support brackets [3] into the perfora- tions [1] on the support profiles with the support bar facing down. -
Page 28: Fig. 15 Center Support Installation
® HERAcell 240 CO Incubator Operating Instructions Initial Setup Figure 15 Center Support Installation: Fig. 15, Center support installation If the CO incubator is equipped with the optional divi- ded insert trays, tow center supports [1] with perfora- tions are installed in addition to the support profiles on the sides. -
Page 29: Gas Connection
1. Push the gas pressure tube [1] onto the gas HERAcell 240 - Gas consumption CO2 (Gas inlet pressure: 1 bar, Amount of gas supplied: 6 l / min, supply connection piece. -
Page 30: Power Connection
® HERAcell 240 CO Incubator Operating Instructions Initial Setup Power Connection Figure 17 WARNING – Electrical shock hazard: Fig.. 17, Power connection Contact with components under electrical power can result in a life-threatening elec- trical shock. Inspect the plug and power cord for da- mage before connecting the unit to the mains power supply. -
Page 31: Rs 232 Interface Connection
® HERAcell 240 CO Incubator Operating Instructions Initial Setup RS 232 Interface Connection Figure 18 The RS 232 interface is designed to accept a cable Fig. 18, RS 232 interface with a 9-pin plug and 1:1 contacts. Unit connection: 1. Switch the PC off. 2. - Page 32 ® HERAcell 240 CO Incubator Operating Instructions Initial Setup Command list – Read Regulatory Circuit Data The entry in index 6 belongs to a higher-level error Command: which appears on all displays (e.g., error code 99). 0110 0001 (61 The error codes for incubation temperature and de- Data: contamination temperature appear on the temperature 0001 0000 (10...
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Page 33: Alarm Contact Connection
Figure 19 NOTE – Specialized tasks: A connector [5] is supplied to allow connection to an Kendro Laboratory Products only warrants the alarm system (cable is not supplied). The table lists safety and proper operability if the installation and re- the values for the operating voltage and fuses on the pair tasks are performed professionally. - Page 34 ® HERAcell 240 CO Incubator Operating Instructions Initial Setup ® HERAcell Alarm Relay Operating mode Contact 4 - 1 Contact 4 - 3 No error, mains off No error, mains on Error X: contact closed / O: contact open Note – Circuit structure: The alarm relay is activated by all errors re- ported by the unit (probe break, setpoint exceeded/not reached, and door open longer than 10 minutes).
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Page 35: Cleaning
® HERAcell 240 CO Incubator Operating Instructions Initial Setup Cleaning Note – Cleaning: The unit must be cleaned before it can be operated. We recommend performing the ContraCon deconta- Chapter 10 contains detailed instructions re- mination routine after cleaning the sample chamber. garding cleaning and disinfecting the unit. -
Page 36: Control, Displays, Indicators
® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Main Power Switch Figure 20 / 21 Depending on the location of the door stop, the main Fig. 20, Switch the unit on/off power switch is integrated into one of the two front feet on the front cover [1]. -
Page 37: Microprocessor Self-Test
® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Microprocessor Self-test After the unit has been switched on at the main power switch, the microprocessor performs a self-test routine. Instruction Operator action Display / explanation 1. Switch the unit on. Press the main power switch. -
Page 38: Temperature Setpoint Specification
® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Temperature Setpoint Specification Instruction Operator action Display / explanation 1. Display setpoint. Press the key. °C The current setpoint is shown on the tempe- rature display. 2. Enter new setpoint. Press the keys, Increase the setpoint. -
Page 39: Setting The High/Low Humidity
® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Setting the High/Low Humidity Instruction Operator action Display / explanation 1. Activate the configuration Press the key for 5 sec. All indicators on the control panel flash. mode. 2. Display the mode. Press the key. -
Page 40: Auto-Start Activation
® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Auto-start Activation The auto-start function is an automated routine to start the unit and subsequently equalize the CO measurement system. After starting, the system controller regulates the defined temperature setpoint. Simultaneously, the humi- dity is built up. -
Page 41: Error Codes
This error table can be recalled for viewing. If you are unable to rectify the cause of the error yourself, contact the Kendro Service and have the error code in question together with your unit’s ID no. ready NOTE –... - Page 42 ® HERAcell 240 CO Incubator Operating Instructions Control, Displays, Indicators Error Codes Instruction Operator action Display / explanation The audible alarm sounds. 2. Shut off the audible alarm. Press any key. The audible alarm stops. 3. Query the error code. Press and hold the key.
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Page 43: Error Code Overview
Contact the Kendro Service. point Temperature above set- Actual value > setpoint +1° C Make sure ambient temperature is below point. permissible maximum. Contact the Kendro Service. Temperature probe Probe break / short circuit Contact the Kendro Service. faulty below setpoint Actual value <... -
Page 44: Resetting The Overtemperature Protector
If the cause cannot be corrected by simple measures such as increasing the room ventilation or reducing the ambient temperature, the overtemperature protector will immediately activate again. In this case, contact the Kendro Service. - 44 -... -
Page 45: Operation
® HERAcell 240 CO Incubator Operating Instructions Operation Preparing the Unit Beginning Operation The unit may only be released for actual operation if Starting and loading the unit: all the measures for initial setup (Sec. 6.1 – 6.7) have 1. Add a maximum of 4.5 l of treated water to the been properly carried out. -
Page 46: Decommissioning
Taking the Unit Out of Service CAUTION – Contamination Hazard! NOTE – Recycling-Service: Kendro Laboratory Products offers a proper recycling service for old equipment. For further infor- The sample chamber surfaces may be mation regarding this fee-based service, please con- contaminated. -
Page 47: Cleaning And Disinfection
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection 10.1 Preparation Users must be aware of all characteristics of materials Perform the following preparatory tasks for a general used and the hazards associated with them. cleaning: • Remove culture vessels and all other items from The operator must provide hygienic guidelines for cle- the sample chamber. -
Page 48: Fig. 22 Protective Screen/Fan Wheel Disassembly
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection Figure 22 Protective Screen and Fan Wheel Removal: Fig. 22, Protective screen/fan wheel disassembly The fan wheel and its protective screen can be disassembled if required. 1. Using the provided Allen wrench (3 mm), remove the two fastening screws [3] on the protective screen, then remove the screen. -
Page 49: Cleaning
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection 10.2 Cleaning 10.3 Manual Disinfection CAUTION – Hazardous fluids: The unit can be disinfected by hand using a disinfec- ting rinse/spray. The rack system components as well as the fan wheel Certain unit components are made of pla- and its protective screen can be autoclaved. - Page 50 10. Cleaning and Disinfection Sample Chamber Disinfection: Recommended Disinfecting Agents: 1. Wipe or spray down all surfaces with a disinfec- Kendro Laboratory Products recommends using Bar- ting agent. rycidal 36 for disinfection. 2. Allow the agent to work according to the manu- Properly employed, Barrycidal 36 represents a highly facturer’s instructions.
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Page 51: Contracon Decontamination Routine
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection 10.4 ContraCon Decontamination Routine The ContraCon decontamination routine decon- The ContraCon decontamination routine requires ap- taminates the entire sample chamber including all prox. 25 hours to run a complete cycle. installed components ands sensors in a single, 1. -
Page 52: Fig. 23 Schematic Diagram Of The Decontamination Routine Process
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection Figure 23 The ContraCon Decontamination Routine: If the door is opened during this phase, the deconta- The routine is subdivided into four phases. Each of mination routine will automatically restart once the these phases can be interrupted individually and thus door is closed again. -
Page 53: Contracon Decontamination Routine Activation
® HERAcell 240 CO Incubator Operating Instructions 10. Cleaning and Disinfection 10.5 ContraCon Decontamination Routine Activation Instruction Operator action Display / explanation 1. Switch the unit on. Press the main power switch. All displays on the control panel go on, the software version is shown on the temperature display and on the am CO display. -
Page 54: Maintenance
Electrical safety inspection in accordance with the NOTE – Maintenance agreement: applicable regulations (e.g., VBG 4). Kendro Laboratory Products offers a mainte- nance agreement tailored to your specific unit and co- NOTE – Functional test: vering all required inspection and maintenance tasks. -
Page 55: Checking The Temperature
® HERAcell 240 CO Incubator Operating Instructions 11. Maintenance 11.3 Checking the Temperature Figure 24 In order to determine the precise value being measu- Fig. 24, Temperature equalization red by the internal temperature sensor, a comparative temperature measurement should be performed quarterly. -
Page 56: Equalizing The Temperature
® HERAcell 240 CO Incubator Operating Instructions 11. Maintenance 11.4 Equalizing the Temperature Instruction Operator action Display / explanation 1. Activate the equalization Press the key for 5 se- All indicators on the control panel flash. function. conds. 2. Display the setpoint. Press the key. -
Page 57: Checking The Co Concentration
A calibrated instrument offering an accuracy of ≤ ± 0.3 % CO should be employed for the compari- son. A suitable Infrared (IR) meter is available from Kendro: • Portable IR meter. (Refer to Chapter 12, “Spare Parts and Accessories” for the order number.) The sample is taken via the measurement opening in the glass door. -
Page 58: Equalizing The Co Concentration
® HERAcell 240 CO Incubator Operating Instructions 11. Maintenance 11.6 Equalizing the CO Concentration Instruction Operator action Display / explanation 1. Activate the equalization Press the key for All indicators on the control panel flash. function. 5 seconds 2. Display the setpoint. Press the key. -
Page 59: Gas Supply
® HERAcell 240 CO Incubator Operating Instructions 11. Maintenance 11.7 Sterile Filter Replacement for the Figure 26 Gas Supply Fig. 26, Sterile filter replacement The sterile filter has plastic threads and is screwed into the socket on the circuit cabinet. 1. -
Page 60: Sterile Filter Replacement For The Co 11.8 Unit Fuse Replacement
® HERAcell 240 CO Incubator Operating Instructions 11. Maintenance 11.8 Unit Fuse Replacement Figure 27 The two identical unit fuses [4] are located in the fuse Fig. 27, Unit fuse replacement box [1] next to the unit socket. The fuse characteri- stics are: •... -
Page 61: Spare Parts And Accessories
12.1 List of Spare Parts and Accessories NOTE – Maintenance: Only original spare parts and accessories approved by Kendro Laboratory Products may be used. Use of other parts may present unknown risks and will result in the warranty being voided. - Page 62 ® HERAcell 240 CO Incubator Operating Instructions 12. Spare Parts and Accessories Designation Type Order no. Fan wheel Stainless steel 50049692 Fan wheel Copper 50051184 Unit fuse T 6.3 A (2) 03002641 Power cord 50043143 Power cord 50047100 Power cord 50047101 Power cord 50047099...
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Page 63: Technical Specifications
® HERAcell 240 CO Incubator Operating Instructions 13. Technical Specifications Designation Unit of measure Value Mechanical Exterior dimensions (W x H x D) 780 x 934 x 834 Internal dimensions (W x H x D) 607 x 670 x 583 Sample chamber volume approx. - Page 64 % rH 80, max. Setup elevation m above sea level 2000, max. HERAcell 240 - Gas consumption CO2 (Gas inlet pressure: 1 bar, Amount of gas supplied: 6 l / min, glass door closed) CO2 concentration [% CO2] - 64 -...
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Page 65: Materials Employed
® HERAcell 240 CO Incubator Operating Instructions 14. Materials Employed Component Material Thermal insulation elements Polystyrene foam, EPS/PPS compound Electronics PCBs Jacketed electronic components with various plastics mounted on epoxy resin bonded circuit boards Plastic components, general Note material identification marks Exterior housing Galvanized sheet steel, painted Rear unit wall... -
Page 66: A Principles Of Good Microbiology Techniques
® HERAcell 240 CO Incubator Operating Instructions Attachment A: Principles of Good Microbiology Techniques General Rules: The following additional basic rules apply when • All doors and windows in the work area should be working with pathogens: • closed while work is in progress. All work areas are to be disinfected daily. - Page 67 ® HERAcell 240 CO Incubator Operating Instructions Attachment A: Principles of Good Microbiology Techniques Depending on the potential risk involved, other The following additional rules apply when hand- safety measures may include: ling human or animal pathogens: • • The use of safety workbenches (with the air flow A permit in accordance with the Federal Epidemic directed away from the individual working at the Law is required to handle human pathogenic bio-...
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Page 68: B Unit Log
® HERAcell 240 CO Incubator Operating Instructions Attachment B: Unit Log NOTE – Unit log: Enter the nameplate information, and any work, maintenance, and repairs carried out here. Unit type: Order number Manufacturer’s number: Service number: Setup location: Operator’s comment: Work performed Comments Date... - Page 69 ® HERAcell 240 CO Incubator Operating Instructions - 69 - Version: 03.2001 / 50065640...
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Page 70: Addresses
Tel. +852 2711-3910 · Fax +852 2711-3858 · info@kendro.com Australia and other North Pacific Asian Regions Kendro Laboratory Products · Unit 4 · 2-6 Orion Road · Lane Cove, Sydney, NSW 2066 · Tel. +61 (02) 9936 1540 · Fax +61 (02) 9427 9765 · info@kendro.com.au China Kendro Laboratory Products Beijing Representative Office ·... - Page 71 Kendro Laboratory Products (India) Pvt. Ltd. · B-5/75 (LGF), Safdarjung Enclave · New Delhi 110 029 · Tel. +91 (11) 618 48 40 · Fax +91 (11) 618 53 97 · kendro.india@vsnl.com Japan Nippon Kendro Co. Ltd. · Muroichi Building, 4/F · 1-13-4 Muromachi, Nihonbashi ·...
- Page 72 ® HERAcell 240 CO Incubator Operating Instructions - 72 - Version: 03.2001 / 50065640...