Fluidics; Fluorescence Sensitivity - Cytek Biosciences Aurora User Manual

Fluidics

Item
General operation
Compatible tubes
Fluidic reservoirs
Sample flow rates
Data acquisition rate

Fluorescence Sensitivity

Item
Fluorescence sensitivity
threshold
Fluorescence linearity
Forward and side scatter
sensitivity
Forward and side scatter
resolution
Side scatter resolution
88 Aurora User's Guide
Specification
Vacuum driven fluidics with the following fluidics modes: Long Clean,
SIT Flush, Purge Filter, Degas Flow Cell, Clean Flow Cell, Fluidics
Shutdown.
12 x 75-mm polystyrene and polypropylene tubes
4-L fluid tanks with level-sensing provided. Compatible with 20-L
sheath and waste cubitainers.
Three preset flow rates:
• Low: 15 μL/min
• Medium: 30 μL/min
• High: 60 μL/min
Up to 35,000 events/s
Specification
FITC: 100 molecules of equivalent soluble fluorophore (MEFL-FITC)
PE: 30 molecules of equivalent soluble fluorophore (MEFL-PE)
APC: 15 molecules of equivalent soluble fluorophore (MEFL-APC)
Pacific Blue: 200 molecules of equivalent soluble fluorophore (MEFL-
Pacific Blue)
NOTE: Measurements performed using SPHERO Rainbow Calibration
Particle (RCP-30-5A) based on its single-peak detection channel.
2
FITC R 0.995 / PE R
≥
Enables separation of fixed platelets from noise.
Performance is optimized for resolving lymphocytes, monocytes, and
granulocytes, as well as microparticles.
Capable of resolving 0.2-μm beads from noise.
2
0.995
≥