Cytek Biosciences Aurora User Manual page 44

Use the Identify Positive/Negative Populations window to include the positive and negative
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populations for each fluorescent tag in the appropriate gate.
a. Move the polygon gate in the FSC vs SSC plot to include the singlet population. Hold down
CTRL to move all the polygon gates at once.
b. Move the interval gate on the spectrum plot on the right to select the channel that exhibits
the brightest fluorescence intensity. This channel is the peak emission channel for the
fluorescent tag.
c. Move the interval gate in the histogram for the peak channel labeled Positive to include the
positively stained population. Move the interval gate in the histogram for the peak channel
labeled Negative to include the negative population.
d. Select Live Unmixing.
The wizard closes and the Acquisition workspace reappears. The Reference Group now has the
unmixed icon to the left of the tube and a new unmixed worksheet appears.
44 Aurora User's Guide