Cytek Biosciences Aurora User Manual page 26

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NOTE: The list of fluorescent tags can be edited in the Library. You can use the Library to add
fluorescent tags that are not present in the default list. See "Fluorescent Tags" on page 55 for
more information.
Define the control type for the fluorescent tags, as well as the unstained controls. Once the
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controls have been defined, select Next.
Either beads or cells can be stained and defined as control types. This allows you to keep track
of control types. If any of the fluorescent tag controls lack a negative population and are of the
same type as the unstained control, check the Universal Negative checkbox at the right.
(Optional) Enter labels associated with the fluorescent tag for identification and tracking.
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5 If necessary, adjust gain settings. Place the appropriate sample on the SIP and click Start to view
the data.
Gain settings for all channels can be selected from the User Settings drop-down menu, or they
can be individually adjusted for each channel using the detector gain spinboxes (V1-V16, B1-
B14, and R1–R8).
The Adjust Settings screen allows you to view the data to ensure that the positively stained
fluorescent particles are not off scale. FSC gain can be adjusted from 0-1000. SSC and detector
channel gain can be adjusted from 0-10,000. If the positive population is off-scale for any
detector channels, lower the gain setting for that channel. If the positive population is not
sufficiently separated from the negative population within a specific channel, adjust the gain
setting for that channel.
26 Aurora User's Guide