Remove the tube of 10% bleach from the SIP and replace with a tube of 3 mL of DI water.
8
Proceed with the Long Clean in the software.
9
When prompted, remove the long clean tubing assembly and re-install the sheath filter.
10
Cleaning the External Surfaces
Periodically check for saline residue.
Dampen a cloth with a mild cleaning solution and wipe the surfaces of the instrument.
1
Dampen a cloth with DI water and wipe the surfaces again to remove residual cleaning
2
solution.
Dry the surfaces with a clean, dry cloth.
3
Inspecting the Fluidics Lines
Check the cytometer periodically for fluid leaks. If any evidence of a leak is detected,
contact Cytek Technical Support immediately. Do not attempt to repair the instrument.
Visually inspect for fluid leaks by looking for small pools of liquid near any of the quick-connects.
1
Visually inspect for dried residue or slight discoloration in the spaces surrounding the
2
cytometer.
Replacing the Sheath Filter
The sheath filter traps debris and air bubbles before they reach the flow cell. Replace the filter
assembly every 6 months or when you see increased debris in an FSC vs SSC plot.
Wear appropriate safety attire such as protective gloves, lab coat, and eyewear while
performing this procedure.
Turn off the cytometer.
1
Open the front cytometer panel.
2
Chapter 8: Maintenance
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