Shutting Down The System - Cytek Biosciences Aurora User Manual

Check the sheath and waste level checkmark indicators. Ensure the status checkmark indicators
5
for sheath and waste are green before proceeding.
Fluid Indicator
Yellow sheath indicator
Red sheath indicator
Yellow waste indicator
Red waste indicator
The cytometer is now ready for Daily QC. See "Performing Daily QC" on page 21.
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Shutting Down the System

The shutdown procedure flushes the flow cell and sample lines with bleach and DI water. The
software provides instructions during the shutdown procedure. The SIT will remain protracted
from the SIP at the end of the shutdown procedure to ensure the SIT does not dry and form clogs.
In the Cytometer menu from either the QC & Setup or Acquisition workspace, select Fluidics
1
Shutdown.
2 Place a tube containing 3 mL of 10% bleach on the SIP. Once loaded, the instrument begins
drawing in the tube contents. This takes approximately 2 minutes.
When prompted, remove the tube and place a tube containing 3 mL of DI water on the SIP.
3
Once loaded, the instrument begins drawing in the tube contents. This takes approximately
2 minutes.
Leave the tube of DI water on the SIP. Make sure the SIT is submerged in the DI water at the end
4
of the Fluidics Shutdown procedure.
Exit SpectroFlo software by clicking the X in the upper-right corner of the application window.
5
Turn off the cytometer and workstation.
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20 Aurora User's Guide
Meaning
Sheath tank is low and requires refilling (see "Filling the Sheath" on
page 17).
Sheath tank is empty and requires refilling (see "Filling the Sheath"
on page 17).
Waste tank is nearing capacity and requires emptying (see
"Emptying the Waste" on page 18).
Waste tank is full and requires emptying (see "Emptying the
Waste" on page 18).