Chapter 10: Glossary - Cytek Biosciences Aurora User Manual

auto-fluorescence
compensation
data file
deconvolve
detector
dot plot
electronic noise
event rate
FCS
filter
The inherent fluorescence arising primarily from cell structures such as
mitochondria and lysosomes. Auto-fluorescence can hinder detection
of dim fluorescent signals.
The process by which spillover fluorescence from secondary
parameters is accounted for so that fluorescence values for a
parameter represent only the fluorescence of the primary fluorophore.
A collection of measured values from a single sample combined with
text describing the data that has been stored as a flow cytometry
standard (.fcs) file to disk.
An algorithm-based process used to reverse the effects of convolution
(or overlapping) on recorded data.
A device that responds to a specific stimulus. Photodiodes and
photomultiplier tubes are two types of detectors in cytometers. They
convert light signals into electronic signals.
A graphical representation of two-parameter data. Each axis of a plot
displays values of one parameter.
Random fluctuation in electronic signals, a characteristic of all
electronic circuits.
The rate at which cells or particles are acquired.
Flow cytometry standard, a standard format for flow cytometry data
files.
An optical device that blocks the passage of part of the incident light,
allowing the rest to pass virtually unchanged.
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Glossary

Chapter 10: Glossary

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