Chapter 6: Advanced Unmixing; Unmixing In The Analysis Workspace - Cytek Biosciences Aurora User Manual

Unmixing in the Analysis Workspace

Post-acquisition unmixing with raw FCS files can be performed in the Acquisition workspace and
can also be performed in the Analysis workspace. You can pick and choose which FCS files to unmix
in the Analysis workspace (for example controls coming from different experiments or single-
stained controls that were not run as part of the Reference Group). In contrast, the unmixing
wizard in the Acquisition workspace limits the FCS files to be used as controls to those coming from
the Reference Group of the Reference Control Library.
In addition, raw FCS files can also be conventionally compensated in this workspace through the
Virtual Filters tab. This function can simulate the presence of filters and can compensate data using
conventional methods that result in output like that obtained from a conventional cytometer.
FCS files can be designated into three categories:
• Single Stained
• Unstained
• Sample

NOTE: There must be at least one single-stained FCS file and one unstained FCS file in the file list.
Otherwise, unmixing cannot be performed.
To Unmix Raw Data Files:
1 Click Import to import raw FCS files for analysis.
Advanced Unmixing

Chapter 6: Advanced Unmixing

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