Virtual Filters - Cytek Biosciences Aurora User Manual

Virtual Filters

Raw FCS data can be compensated using conventional methods in the Virtual Filter tab.
1 Click the Virtual Filter tab in the Analysis workspace.
Select Import to import raw FCS files for virtual filter analysis.
2
These FCS files can be single-stained Reference Control FCS files, unstained control FCS files,
and/or sample FCS files. It is important to note that an unstained control FCS file must be
included.
3 Upon importing, a dialog box on how to assign sample types appears. Read the instructions
and click OK.
Once FCS files have been imported, the sample type for each FCS file needs to be designated as
4
Single Stained, Unstained, or Sample. The software will automatically designate the type based
upon the file name. This can be modified manually if the automatic designation is incorrect.
FCS files designated as single stained will require a fluorescent tag designation. If there is no
negative population in the single-stained FCS file, select the Universal Negative option.
Chapter 6: Advanced Unmixing 51