Post-Acquisition Unmixing - Cytek Biosciences Aurora User Manual

Post-Acquisition Unmixing

Samples can be acquired as raw data and then unmixed after acquisition is complete in the
experiment. This can be done through two methods:
• post-acquisition unmixing in the Acquisition workspace
• post-acquisition unmixing in the Analysis workspace
Post-Acquisition Unmixing in the Acquisition workspace
To perform post-acquisition unmixing in the Acquisition workspace, perform the same workflow as
live unmixing EXCEPT the following:
Acquire all Reference Control tubes and sample tubes prior to selecting the unmix button in the
1
upper-left pane.
Select Unmix, Save & Open.
2
Experiments that have been unmixed post-acquisition can be found in the Unmixed tab of the
3
My Experiments menu.
Post-Acquisition Unmixing in the Analysis workspace
To perform post-acquisition unmixing in the Analysis workspace, see "Unmixing in the Analysis
Workspace" on page 47.

NOTE: If one of the controls is questionable, you can reacquire it, overwriting the original file,
then unmix again.
Chapter 5: Acquisition 45