Instrument Setup - Reference Controls; Creating Reference Controls - Cytek Biosciences Aurora User Manual

Instrument Setup - Reference Controls

Reference Controls must be acquired and recorded to ensure accurate spectral unmixing of the
data. References are obtained by acquiring particles stained with individual fluorescent tags. Either
beads or cells can be used as single-stained controls for acquiring references. You can select
whether to create new Reference Controls or update Reference Controls already stored in the
Library.
• Reference Control - references stored and retrieved from the Library
• Reference Group - references acquired and recorded in the experiment
A step-by-step wizard guide you through recording Reference Controls.

Creating Reference Controls

To create Reference Controls you will need to define the fluorescent tags, define the controls, then
label the fluorescent tags.
Select New Reference Controls from the Reference Controls tab in the QC & Setup workspace.
1
A wizard opens allowing you to create new Reference Controls.
Select fluorescent tags. The left pane displays the fluorescent tag groups found in the Library.
2
• Click the arrow to the left of the fluorescent tag group name to view the fluorescent tags
associated with the group. (The default fluorescent tag groups are Blue Laser, Red Laser, and
Violet Laser and contain a list of commonly used fluorescent tags excited by their respective
lasers).
• From the expanded list of fluorescent tags, select the fluorescent tags used in the
experiment. Once selected the fluorescent tags appear in the selection pane on the right side
of the Define Fluorescent Tags window. You can select fluorescent tags by dragging and
dropping, double-clicking, or using the Add button. Multiple tags can be chosen at one time.
Confirm the tags selected, then click Next.
Chapter 4: QC & Setup 25