Chapter 11: Specifications; Cytometer; Optics - Cytek Biosciences Aurora User Manual

Cytometer

Optics

Item
Optical platform
Lasers
Beam geometry
Emission collection
Forward scatter detector
and filter
Violet side scatter
detector
Fluorescence detectors
Standard optical
configuration
Specification
Fixed optical assembly configured with three spatially separated laser
beams. Laser delays are automatically adjusted during instrument QC.
405 nm: 100 mW
488 nm: 50 mW
640 nm: 80 mW
Flat-top laser beam profile with narrow vertical beam height
optimized for small particle detection.
Fused silica cuvette coupled to high NA lens for optimum collection
efficiency to optical fibers.
High-performance semiconductor detector with 488-nm bandpass
filter
High-performance semiconductor detector with 405-nm bandpass
filter
Proprietary high-sensitivity Coarse Wavelength Division Multiplexing
(CWDM), 16-channel semiconductor detector array per laser, enabling
more efficient spectrum capture for dyes emitting in the 400-nm to
900-nm range. No filter changes required for any fluorophore excited
by the 405-nm, 488-nm, and 640-nm lasers.
Violet detector module: 16 channels uneven spaced bandwidth from
420 nm–830 nm.
Blue detector module: 14 channels uneven spaced bandwidth from
500 nm to 890 nm. Up to 16 channels available.
Red detector module: 9 channels uneven spaced bandwidth from
650 nm to 890 nm standard. Up to 16 channels available.
Specifications

Chapter 11: Specifications

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