Replacing The Sit - Cytek Biosciences Aurora User Manual

Press the two fluidics line quick-connects and the vent line quick-connect to the right of the
3
sheath filter.
Figure 11. Sheath filter and sheath filter quick-connects
4 Disconnect the vent line.
5 Discard the filter according to standard laboratory protocol and local regulations.
6 Install a new sheath filter with the arrow pointing up.
Run the Purge Filter fluidic mode to remove air bubbles (see "Purging the Sheath Filter" on
7
page 72). Repeat this step until all air bubbles are purged from the filter.
Close the front panel.
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Replacing the SIT

Replace the SIT if the tubing is clogged even after repeatedly cleaning and flushing the SIT.
Ensure the SIT is protracted in a tube of DI water and the cytometer is turned off.
1
If the cytometer was shut down properly using the Fluidics Shutdown procedure, the SIT will
already be protracted and left is water.

NOTE: If the SIT is not protracted, turn on the cytometer and run Fluidics Shutdown. Then
turn off the cytometer.
Obtain a SIT tubing assembly.
2
Open the SIT door. Identify the four components shown below.
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