Emptying The Waste - Cytek Biosciences Aurora User Manual

Filling Sheath into a Cytek 4-L Sheath Tank or a 20-L Cubitainer:
Remove the sheath fluidic line cap from the cubitainer or sheath tank lid from the Cytek sheath
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tank.
2 Add the appropriate sheath solution.
Replace the fluidic line cap or sheath tank lid. Do not over-tighten.
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4 If the cytometer is powered on and the software is connected, verify that the software sheath
indicator is green.

Emptying the Waste

Waste can be expelled into either the supplied 4-L waste tank or directly into an empty 20-L
cubitainer.
Empty the waste container when filling the sheath container or as needed to prevent
leakage. The software indicator for waste will be yellow or red when the container needs
to be emptied. Take care to avoid damaging the fluid level sensor in the waste tank.
Biological samples are potentially dangerous and/or life threatening. Adhere to proper
handling procedures for samples and reagents. Wear appropriate laboratory attire such
as protective gloves, eyewear, and lab coat during this procedure.
Always treat the contents of the waste container with bleach (10% of the total volume).
Contents of the waste container may contain biohazardous material.
Removing Waste from a Cytek 4-L Waste Tank or a 20-L Cubitainer:
Disconnect the waste line quick-connect from the front of the cytometer.
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Disconnect the waste line orange quick-connect from the cubitainer cap or 4-L waste bottle.
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Disconnect the waste level sensor.
The waste level sensor connector for the cubitainer is on the cubitainer cap. The waste level
sensor connector for the 4-L tank is on the front of the cytometer.
Remove the waste cap from the cubitainer or the lid from the 4-L waste tank, taking care not to
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damage the liquid level sensor.
4 Dispose of the waste per local regulations.
5 Add 2 L of undiluted bleach to the waste cubitainer, or 400 mL of bleach to the waste tank.
6 Replace the waste cap/lid to the container. Hand-tighten the cap/lid until it is fully closed.
Reattach the waste line and level sensor line to the cap/lid and front of the cytometer.
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8 If the cytometer is powered on and the software is connected, verify that the software waste
indicator is green.
18 Aurora User's Guide