Cytek Biosciences Aurora User Manual page 53

Select Show Plots to display the plots.
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The data is displayed in the FSC vs SSC plot and fluorescent tag histogram plot. The positive and
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negative populations need to be identified through the appropriate placement of the gates.
a. Move the polygon gate in the FSC vs SSC plot to include the singlet population.
b. Move the interval gate in the histogram labeled Positive to include the positively stained
population. Move the interval gate in the histogram labeled Negative to include the negative
population. Do not adjust the negative gate when using the Universal Negative.
Chapter 6: Advanced Unmixing 53