Cytek Biosciences Aurora User Manual page 28

During acquisition the spectra plot for each fluorescent control is displayed. The plots show all
the channels across all lasers in the x-axis vs mean fluorescence intensity (MFI) of the
fluorescent tag.
During acquisition obtain spectral information by moving the polygon gate on the FSC-A vs
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SSC-A plot to include the population of interest.
Hold down the Ctrl key while adjusting the gate to move the polygon gates for all the
fluorescent tags at once. The gated population appears in the histogram, which is set to the
peak emission channel of the fluorescent tag to be acquired. The emission spectrum of the
population is displayed in the spectrum plot.
Adjust the positive gate on the histogram. The software automatically displays the emission
spectrum of the positive particle in the spectrum plot. SpectroFlo software sets the default gate
on the peak emission channel. The gate can be selected manually. It is best to set the gate on
the brightest emission as this can make distinguishing the positive and negative population
easier.
Readjust the positive and/or negative gate on the histogram, if necessary.
Select Save to save the Reference Controls to the Library.
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28 Aurora User's Guide