Chapter 4: Qc & Setup; Daily Qc; Performing Daily Qc - Cytek Biosciences Aurora User Manual

Daily QC

Run Daily QC using SpectroFlo QC beads prior to acquiring samples to ensure that the cytometer is
performing optimally. Daily QC assesses the instrument's optical alignment and the system
performance drift by measuring rCVs and gain needed to place the beads at the target locations for
each detector. During QC, laser delays and area scaling factors are optimized and gain settings are
adjusted to ensure day-to-day repeatability. Upon completion of Daily QC, a QC report is generated.
QC reports can be reviewed under the Reports tab.
Performance can be tracked and charted over time in the Levey-Jennings tab. The software can be
configured to display a warning if the QC result on the QC report exceeds user-defined criteria. See
"Alarm Ranges" on page 30.

Performing Daily QC

Daily QC ensures that the instrument is performing optimally. Instrument performance is
characterized and tracked, laser delays and area scaling factors are determined, and user gain
settings are adjusted to account for day-to-day instrument variability.
Allow at least 30 minutes to elapse after turning on the instrument to ensure the lasers are
1
warmed up.
2 Load a 12 x 75-mm tube of SpectroFlo QC beads (1 drop in 0.5 mL sheath, PBS, or DI water) onto
the SIP.
The SpectroFlo QC beads are hard dyed polystyrene beads that have a single fluorescence
intensity. They can be excited by each laser and emit fluorescence in all detector channels.
QC & Setup
Chapter 4: QC & Setup
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