Decontaminating The Fluidics System - Cytek Biosciences Aurora User Manual

Decontaminating the Fluidics System

Decontaminate the fluidics system monthly by running the Long Clean fluidic mode. Run the Long
Clean just prior to service calls and if you run high volumes of unwashed samples or samples
stained with propidium iodide, acridine orange, or thiazole orange.
Do not run bleach or detergent through the sheath filter. This can damage the filter,
resulting in paper fragments that could clog the flow cell.
In the Cytometer tab, from either the QC & Setup or Acquisition workspace, select Long Clean.
1
Follow the instructions that appear. Prepare the appropriate cleaning tubes and fluidic tanks.
2
Empty the waste tank. Replace the sheath filter with the sheath filter bypass (long clean tubing)
3
assembly.
Figure 10. Long clean tubing assembly connected in place of sheath filter
Detach the sheath tank and replace it with a tank containing a 10% bleach solution.
4
Install a tube containing 3 mL of a 10% bleach solution on the SIP.
5
Proceed with the Long Clean in the software.
6
Once the bleach cleaning cycle is complete, reattach the sheath tank.
7
74 Aurora User's Guide