Qc Report - Cytek Biosciences Aurora User Manual

When Daily QC passes, the following message is displayed.
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You are now ready to acquire samples.

QC Report

At the completion of the Daily QC run, a QC report is generated. The report includes the following
sections:
• The header section contains the name of the instrument, date the Daily QC was run, user who
ran the Daily QC, instrument configuration, instrument serial number, SpectroFlo QC bead lot
and expiration date, and Pass/Fail status of the run.
• The results section contains the gain, gain change, median fluorescent intensity of the daily QC
bead, %rCV, and a pass/fail indicator for each detector channel. The center wavelength of the
detector is shown in parentheses next to the detector name.
• The Laser Settings section contains the laser delays for all non-primary lasers, and area scaling
factors for all lasers and the FSC detector
Pass/Fail Criteria - The pass/fail criteria are the following:
• %rCV must not exceed 6% for the FSC channel
• %rCV must not exceed 8% for the SSC channel
• %rCV must not exceed 6% for the V3 channel
• %rCV must not exceed 6% for the B3 channel
• %rCV must not exceed 6% for the R3 channel
• Delta gain for all channels must not exceed 100 from the last Daily QC run performed by Cytek
Service personnel.
The number of reports listed in the Reports screen can be set in the Preferences. See "QC Setup" on
page 66 for more information.
Chapter 4: QC & Setup 23