Cytometer Overview - Cytek Biosciences Aurora User Manual

Cytometer Overview

The Aurora spectral flow cytometer is an air-cooled, multi-laser, compact benchtop flow cytometer.
It is equipped with three lasers and up to 48 detection channels for fluorescence and up to two
detection channels for scatter (FSC [forward scatter] and violet laser SSC [side scatter]). Solid-state
lasers transmit light through a flow cell where particles in suspension are focused, single file for
interrogation by the laser. Proprietary, high-sensitivity, 16-channel semiconductor detector arrays
are equipped to capture the emission spectra of dyes that emit in the 400 to 900-nm wavelength
range. The resulting fluorescence and scatter are then collected and converted into electronic
signals. On-board electronics convert these signals into digital data that can be acquired and
recorded on the workstation.
The cytometer power button is located on the left side of the upper panel of the cytometer
(Figure 2). When the cytometer is powered on, the power button is illuminated.
The front panel opens on its hinges to the right to reveal the fluidics system. The top cover opens to
reveal the optics.
Front of Cytometer
Figure 2. Aurora front and left side panels
Do not place any object on top of the instrument.
Do not place liquids on top of the instrument. Fluid leaking into the cytometer could
cause electrical shock or damage to the instrument.
10 Aurora User's Guide
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