Cleaning The Sit; Purging The Sheath Filter - Cytek Biosciences Aurora User Manual

Cleaning the SIT

A sample line backflush is performed whenever a tube is removed from the SIP after sample
acquisition. If the sample line exhibits signs of carryover or becomes clogged after completing an
experiment with a sticky dye such as propidium iodine, acridine orange, or thiazole orange, the
sample line should be manually backflushed.
In the Cytometer tab, from either the QC & Setup or Acquisition workspace, select SIT Flush.
1
If carryover or a clog persists, place a tube of 10% bleach on the SIP and acquire at High flow
2
rate for 5 minutes. Afterwards, acquire a tube of DI water at High flow rate for 5 minutes.
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NOTE: If running large quantities of beads or large cells, we recommend running a tube of 10%
bleach followed by a tube of DI water, each for 5 minutes, between experiments.

Purging the Sheath Filter

Perform this procedure if air bubbles are visible in the sheath filter, or if the plenum or
sheath tank have run dry and air is present in the fluidics system.
1 In the Cytometer tab, from either the QC & Setup or Acquisition workspace, select Purge Filter.
The vent valve connected to the sheath filter will open releasing any air bubbles trapped inside
the sheath filter.
Repeat the Purge Filter fluidic mode until there are no visible bubbles inside the sheath filter.
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