Step 5: Start The Run; Zonal And Continuous Flow Operation - Beckman Coulter Optima XE Series User Manual

Step 5: Start the Run

Once the run values are set, start the run:
1
Prepare your samples and place them in the rotor following all proper procedures, including
balanced weight distribution.
2
Preheat or precool the rotor and samples, if necessary.
3
Mount the rotor in the instrument following all the procedures in the rotor manual and
observing all safety procedures and cautions.
4
Close and lock the chamber door. For preconditioning, press the Vacuum Display/Button and
wait until the chamber reaches the set temperature.
5
Press the
Figure 4.7 Start Button
When the run begins, wait for the countdown timer to reach zero and the rotor to come to a stop.
You can then remove your rotor and samples.

Zonal and Continuous Flow Operation

WARNING
In zonal and continuous flow operation, the operator is unavoidably exposed to
rotating machinery. For safety, the operator must be properly instructed and
qualified. Guard against accidentally dropping objects, such as pens, pencils, or
hemostats into the chamber. Loose lab coats, neckties, scarves, and long
necklaces should not be worn while operating in the zonal or continuous flow
mode.
Use only zonal rotors in the zonal mode.
Use only continuous flow rotors in the continuous flow mode.
Zonal and continuous flow operations require special rotors and have some additional hazards in
that samples are loaded and unloaded while the rotor is spinning. To prevent unauthorized users
from attempting these operations, the instrument requires a password (which is 1793). When zonal
PN B07711AA
button.
Start
Operations
Zonal and Continuous Flow Operation
4
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