Purging The Pump - Agilent Technologies 1290 Infinity User Manual

Purging the Pump

When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will re-diffuse
into the solvent channel between the solvent reservoir, vacuum degassing unit
(when available in the system) and the pump. Solvents containing volatile
ingredients will slightly lose these. Therefore purging of the pumping system is
required before starting an application.
1 Initiate a purge in the controlling software with a Purge flow set to
2 Flush all tubes with at least 30 ml of solvent.
Table 6 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse phase and
normal phase (both times)
After an installation
To clean the system when using buffers
After a solvent change
Before turning off system for an extended
period of time
The pump should never be used for priming/purging empty tubings (never let the pump
N O T E
run dry). Use a syringe to draw enough solvent for completely filling the tubings to the
pump inlet before continuing to prime with the pump.
1290 Infinity Quaternary Pump User Manual
3 – 5 ml/min per channel.
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Organic or 10 %
isopropanol in water
Using the Module

Purging the Pump

Comments
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
Alternative to Isopropanol (second choice) if no
Isopropanol is available
Best solvent to re-dissolve buffer crystals
Best solvent to re-dissolve buffer crystals
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