Purging The Pump - Agilent Technologies 1100 Series Service Manual

1 Installing the Pump
5 Connect the end of the solvent tube to the syringe adapter. Slowly draw a
6 Disconnect the solvent tube from the syringe adapter, and reconnect the
7 Repeat steps 4 to 6 for the three remaining solvent channels.
8 When all 4 solvent channels are manually primed, remove the paper towel

Purging the Pump

1 Make sure that the 1/8 inch plastic waste tube is tightly connected to the
2 Turn on the LC System. All system parameters should be set to default. The
3 Initialize the system. Then, access the pump controls and make sure the
4 Access the pump Purge control. Set up a purge table which will purge all
When the pump has been turned off for a certain time (for example, overnight), oxygen will
NO TE
re-diffuse into the channels between the degasser and the pump. It is suggested to purge
each channel at 2500 µl/min for 1 minute at the beginning of each day.
Table 4 Choice of Priming Solvents for Different Purposes
Activity
After an installation
After an installation (second choice)
When switching between reverse phase
and normal phase (both times)
18
syringe volume (20ml) from the solvent tube.
tube to the A1 port of the solvent selection valve. Eject the syringe contents
into an appropriate waste container.
from the pump leak tray. Make sure that the pump leak sensor is dry before
turning on the pump.
barbed waste fitting of the pump EMPV, and routed to an appropriate waste
container.
degasser should also be turned on at this time.
pump mode is set to Normal.
channels for 5 minutes each, at a flow of 2500 µl/min. Then, start the purge.
Solvent
Isopropanol
Ethanol or Methanol
Isopropanol
Comments
Best solvent to flush air out of the system
Alternative to Isopropanol if no Isopropanol
is available
Best solvent to flush air out of the system
1100 Series Nano Pump Service Manual