Method Parameter Settings - Agilent Technologies 1290 Infinity User Manual

4
Using the Module
Setting up the Pump with the Instrument Control Interface

Method Parameter Settings

The Quaternary Pump method setup parameters are in nine sections:
• Flow
• Solvents A to D
• Stoptime
• Posttime
• Pressure Limits
• Timetable
• Advanced
• Blend Assist
• ISET
Table 4 Method parameters
Parameter Limits
Flow 0.00 – 5.00 mL/min in
steps of 0.001 .
Recommended flow
range: 0.05 –
5.00 mL/min .
Enable Blend Assist
Solvents
68
Description
The flow is the rate of movement of eluent along the column. It is important
that the flow rate is kept constant to ensure precise retention time, and
peak measurements. Variations in flow rate can occur as a result of the
partial failure of the pumping system, air in the pumping system, a change
in the mobile phase viscosity or a temperature change.
Mark this check box to switch on Blend Assist, which allows you to set up
solvent mixtures from stock solutions. When this check box is marked, the
Blend Assist section of the method setup is available.
Blend Assist Disabled: When Blend Assist is disabled, you can set the
percentages of solvents B, C and D to any value from 0 through 100 %.
Solvent A always delivers the remaining volume: 100 - (%B + %C + %D).
The check boxes allows you to turn the solvent channels on (checked) or
off (cleared). Click the solvent name down arrow and select the solvent
from the list of calibrated solvents and solvent mixtures. For solvent
mixtures, specify the percentage of additive. You can enter your own name
for the solvent or solvent mixture in the adjacent field.
Blend Assist Enabled: When Blend Assist is enabled, the table shows the
solvent blends that have been set up in the Blend Assist section of the
method setup.
• Solvent: The solvent or blend of solvents as set up in the Blend Assist
section.
• Used: Mark this check box if you want to use this solvent or blend in
the method.
• %: Enter the percentage of the solvent or blend in this field.
• Name: Type a name for the solvent or blend in this field.
1290 Infinity Quaternary Pump User Manual