Purging The Pump - Agilent Technologies InfinityLab LC Series User Manual

3 Using the Pump

Purging the Pump

Purging the Pump

When the solvents have been exchanged or the pumping system has been turned
off for a certain time (for example, overnight) oxygen will re-diffuse into the
solvent channel between the solvent reservoir and the pump. Solvents containing
volatile ingredients will slightly lose these. Therefore purging of the pumping
system is required before starting an application.
Table 4 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse phase and
normal phase (both times)
After an installation
To clean the system when using buffers
After a solvent change
Before turning off system for an extended
period of time
It is recommended not to exceed 50 mL/min flow rate when purging.
NOTE
To purge the pump in LabAdvisor:
1 Prepare each channel with the appropriate purge solvents.
2 Select Purge Pump from the Tool Selection screen.
3 In the Purge Configuration dialog box, if necessary, select the channel(s) that
4 For each selected channel, select a Flow and a purge Time.
Preparative Binary Pumps User Manual
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Organic or 10 %
isopropanol in water
you want to purge.
Comments
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
Alternative to Isopropanol (second choice) if no
Isopropanol is available
Best solvent to re-dissolve buffer crystals
Best solvent to re-dissolve buffer crystals
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