Flow Connections
Capillaries
With the Chip Cube, only PEEK-coated fused silica capillaries are used as they
offer uniform inner diameter, excellent surface smoothness and have a
pressure rating suitable for all nano applications. Fused silica is compatible
with all common HPLC solvents.
Fused silica capillaries are attacked by basic solvents.
C A U T I O N
Do not use solvents with pH > 8.5 in combination with PEEK coated fused silica
capillaries.
Valve Stator
There are six solvent ports on the valve stator of the Chip Cube. Four ports are
currently used by the supplied HPLC-Chips. The attached capillaries can be
left in place regardless of the chip in use. Depending on the type of chip
different ports are used.
port 5
port 6
port 1
Figure 12
The enrichment column of the Protein ID chip can be flushed in either
forward-flush or backflush mode. For details see
page 14.
HPLC-Chip Cube Interface User's Guide
Ports of the Stator Assembly
Introduction to the Chip Cube
Principle of Operation
"Modes of Operation"
2
port 4
port 3
port 2
on
29