Spray Ignition - Agilent Technologies 1260 Infinity User Manual

5 Operation

Spray Ignition

Spray Ignition

Typically, spray ignition does not require any optimization of the chip
position.
Table 8
Parameter
• nanopump
• loading pump
• MS: nitrogen
• MS: N2 temperature
• Capillary voltage
• MS parameters
What a spray looks like depends very much on the solvent composition of the
mobile phase. In general, generating a stable spray is more difficult with
aqueous solvents due to the high surface tension of water. The nanospray
process produces both evaporated ions and charged droplets. The system is
designed to separate and collect only evaporated ions while minimizing the
charged droplets which enter the MS inlet as this approach produces the best
S/N.
66
Typical instrument parameters for igniting nanospray
Value
0.3 µL/min
4 µL/ min
4 L/min
300 deg C
1800 V
Remarks
98/2 water/ACN (both with 0.1% formic acid)
98/2 water/ACN (both with 0.1% formic acid)
increase if necessary in increments of 50 V
see Chip Cube Interface Adapter guide
HPLC-Chip Cube Interface User's Guide