Injector Flush Volume And The Use Of Injector Programs - Agilent Technologies 1260 Infinity User Manual

Typical values are:
For a typical HPLC-Chip Cube system with 75 µm seat capillary, the Injection
Flush Volume is 7.2 µL.
The injector flush volume feature will only work if the inner valve is in Enrichment
C A U T I O N
position at the start of the run.

Injector Flush Volume and the use of Injector Programs

The Chip Cube queries all samplers in the Agilent 1260 Infinity LC stack prior
to the run (to be more precise: before the sampler(s) start to move and draw
sample) and adds the found injection volumes to the Injector Flush Volume.
Changing the injection volume in the sequence table is possible and will be
taken into account.
Example 1: 1260 Infinity LC system with one auto sampler
✔
Example 2: 1260 Infinity LC system with two auto samplers
HPLC-Chip Cube Interface User's Guide
V = delay volume of the capillary from µ-WPS to Chip Cube
TransferCap
factor = compensation factor for hyperbolic flow profile
V 75 µm x 150 mm (blue) = 663 nL
SeatCap
V 100 µm x 150 mm (black) = 1178 nL
SeatCap
V 25 µm x 1050 mm (yellow) = 515 nL
TransferCap
factor = 6
• µWPS: injection volume in method: 1 µL
• Injection volume in sequence table for next run: 5 µL
• Injector Flush Volume: 8 µL
Resulting computed value: 5 µL + 8 µL
• µWPS 1 injection volume in method: 1 µL
• Injection volume in sequence table for next run: no entry
• µWPS 2 injection volume in method: 0.5 µL (e.g. internal Std.)
Operation 5
Configuration
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