Time Table; Instrument Actuals - Agilent Technologies 1260 Infinity User Manual

Time Table

As an alternative to defining of an Injector Flush Volume (see
Volume"
This time table provides full control over the valves, but does not correct
automatically for changes in loading pump flow rate and injection volume.
This mode is intended for experienced users who have used valve timing
before and want to transfer their methods with minimal modifications.
As no adjustment is made when flow rate or injection volume are changed, Agilent
N O T E
recommends using the Injector Flush Volume (see
instead.
Practical Hints
• Use Enrichment as default position for the inner valve.
• Switching to Analysis after 2.5 min has proven to be safe for a loading flow
• It is good practice to switch back to Enrichment before the end of the run.

Instrument Actuals

Turn on the Instrument Actuals display from the View menu in the Method &
Run Control screen.
HPLC-Chip Cube Interface User's Guide
on page 56) a time table can be set up to control the Chip Cube valves.
rate of 4 µL/min and sample volumes of around 1 µL with either 100 µm or
75 µm seat capillaries in the µ-well-plate sampler. For larger sample
volumes, the time will need to be increased.
This ensures that the enrichment column has been equilibrated to high
aqueous mobile phase before the start of the next run.
Setting up Chip Cube Parameters
"Injection Flush
"Injection Flush Volume"
Operation 5
on page 56)
63