Injection Flush Volume - Agilent Technologies 1260 Infinity User Manual

5 Operation
Configuration
Figure 22
Usually, the G1376A capillary pump is used as loading pump and the G2226A
nano pump acts as the analytical pump. Two nano pumps can be used as well.
Select the appropriate pumps from the drop down lists in the configuration
dialog box.
Due to the lack of an electronic purge valve, isocratic, binary and quaternary pumps are
C A U T I O N
not supported. The pressure must be released in a controlled manner prior to unloading
the chip or the chip can be damaged.

Injection Flush Volume

Defining an Injection Flush Volume allows for automated timing of the Chip
Cube injection valve. When set, the G4240A Chip Cube firmware adjusts the
loading time of the enrichment column automatically when flow rates or
injection volumes are changed.
To disable this feature, enter an Injection Flush Volume of 0 µL.
The appropriate Injection Flush Volume can be calculated using the following
equation:
where:
56
Pump Configuration Dialog box
(V + V
SeatCap TransferCap
V = delay volume of the µ-WPS seat capillary
SeatCap
) x factor
HPLC-Chip Cube Interface User's Guide