Operational Parameters - Agilent Technologies 1260 Infinity User Manual

5 Operation

Operational Parameters

Operational Parameters

The table below gives an overview of typical mobile phases, flow rates and
gradients for use with the different HPLC-Chips.
The maximum operation pressure of the HPLC-Chips is 150 bar. This value is
stored in an RFID tag inside the chip holder handle. If the pressure limit of the
Chemstation method is set to a higher value as the maximum operating
pressure of the HPLC-Chip, Chemstation opens a popup box and notifies the
customer.
It is the responsibility of the user to set the upper pressure limit of the ChemStation method
N O T E
in accordance to the installed HPLC-Chip.
If the upper pressure limit of the method is higher than 150 bar, the HPLC-Chip may get
C A U T I O N
damaged permanently if the system pressure rises above 150 bar.
Table 7 Typical Pump Parameters for use with Chip Cube
Flow Rate
• Analytical pump 0.3 µL/min
• Loading pump 4 µL/min
• Infusion pump 18 µL/h
(syringe)
60
Diagnostic & MS Calibration Chip
G4240-61001
A1: water or water with 0.1% formic acid
B1: not used
not used
MS calibrant or clean sample that shall be
infused.
Protein ID Chip G4240-62001
(75 µm x 43 mm, 5 µm C18 SB-ZX)
A1: water with 0.1% formic acid
B1: ACN with 0.1% formic acid
Gradient from 2% - 50% B
A1: water with 0.1% formic acid
B1: ACN with 0.1% formic acid
isocratic at 2% B
Alternative: premixed from one channel
not used, port is not connected to the
chip.
HPLC-Chip Cube Interface User's Guide