Luminescence; Glossary - Molecular Devices SpectraMax M2 User Manual

A. Appendix
A.7.
A.8.
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
56

LUMINESCENCE

Emerald and Emerald II
Sapphire and Sapphire II
a.Emerald, Emerald II, Sapphire, Sapphire II, and Ruby
are trademarks of Tropix, Inc.

GLOSSARY

Absorbance
Absorbance is the amount of light absorbed by a solution. To measure absorbance accu-
rately, it is necessary to eliminate light scatter. In the absence of turbidity, absorbance =
optical density.
A
where I is incident light, and I is transmitted light.
0
In this user guide, we use the terms absorbance and optical density interchangeably.
Automix
The Automix function determines how often, if at all, automated shaking of the micro-
plate is performed during a reading. This feature is covered by U.S. Patent Number
5,112,134.
Emission Cutoff Filter
A long pass filter used to condition the emission light prior to detection by the PMT. In
automatic mode, the instrument sets the cutoffs automatically based upon the wave-
length(s) chosen for reading; in manual mode, you can choose the filter wavelength man-
ually.
Endpoint
A single reading made at one or more excitation/emission wavelengths.
Emission Spectral Scan
Measures fluorescence or luminescence across a spectrum of wavelengths for emitted light
at a fixed excitation wavelength (or no excitation in the case of luminescence). The default
value reported for each well is the wavelength of maximum fluorescence or luminescence.
Fluorophore Wavelength (nm)
a
a
a
Ruby
⁄
( )
= log I I
0
542
461
620