Molecular Devices SpectraMax M2 User Manual page 22

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However, some functionality is available directly on the instrument without having to use
SoftMax Pro:
Temperature control
>
Wavelength control
>
Fixed-point cuvette readings
>
2.8.1.
TEMPERATURE REGULATION
The SpectraMax M2 and SpectraMax M2
temperature of the microplate chamber from 4°C above ambient to 45°C. Upon power
up, when the incubator is off, the temperature in the microplate chamber is ambient and
isothermal. Turning on the incubator by pressing the
instrument to begin warming the microplate chamber. The temperature set point defaults
to 37.0°C at start-up.
Accuracy of the temperature set point is guaranteed only if the set point is at least 4°C
above ambient. If the temperature set point is lower than the ambient temperature, the
chamber temperature remains at ambient. Temperature regulation is controlled by heaters
only and, therefore, cannot cool the temperature to a setting lower than ambient.
Additionally, the highest setting (45°C) can be achieved only if the ambient temperature
is greater than 20°C.
Typically, the microplate chamber reaches 37.0°C in less than 30 minutes. The microplate
chamber temperature is maintained at the set point until you press the incubator
TEMP on/off
Should you turn the incubator back on after a momentary shutdown, allow about ten
minutes for the control algorithm to fully stabilize the microplate chamber temperature.
Temperature regulation and control of the microplate chamber is achieved through
electric heaters, a fan, efficient insulation, and temperature sensors. The heaters are
located in the microplate chamber, which is insulated to maintain the temperature set
point. The sensors are mounted inside the chamber and measure the air temperature.
The temperature feedback closed-loop control algorithms measure the chamber air
temperature, compare it to the temperature set point, and use the difference to calculate
the regulation of the heating cycles. This technique results in accurate, precise control of
the chamber temperature with a temperature variation of the air inside the chamber of less
than 1.0°C. The temperature uniformity within the microplate depends on its design and
composition.
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
key again, turning temperature regulation off.
2.8. Functional Description
e
have been designed to regulate the
key causes the
TEMP on/off
17

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