Related Manuals for Molecular Devices SpectraMax M3
Summary of Contents for Molecular Devices SpectraMax M3
- Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018...
- Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
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Page 3: Table Of Contents
Contents Safety Information Warnings, Cautions, Notes, and Tips Chapter 1: Introduction Computer Integration Supported Plates and Cuvettes Shake Temperature Regulation Chapter 2: Setting Up the Instrument Connecting Instrument Cables Chapter 3: Getting Started Control Panel Cuvette Chamber Plate Drawer Plate Adapter Chapter 4: Operating the Instrument Temperature Settings Wavelength Settings... - Page 4 SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Chapter 7: Troubleshooting Opening the Drawer Manually Obtaining Support Appendix A: Specifications System Diagrams and Dimensions Appendix B: Accessories Appendix C: Electromagnetic Compatibility 0112-0115 G...
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Page 5: Safety Information
Follow the related safety information. The following user attention statements might be displayed in the text of Molecular Devices user documentation. Each statement implies the amount of observation or recommended procedure. - Page 6 For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device. - - California proposition 65 requires businesses to provide warnings to Californians about significant exposures to chemicals that cause cancer, birth defects, or other reproductive harm.
- Page 7 Safety Information Electrical Safety To prevent electrical injuries and property damage, inspect all electrical equipment before use and report all electrical deficiencies. Contact Molecular Devices technical support for equipment service that requires the removal of covers or panels. WARNING! HIGH VOLTAGE.
- Page 8 SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide CAUTION! Use of organic solvents can cause harm to the optics in the instrument. Extreme caution is recommended when you use organic solvents. Always use a plate lid and do not place a plate that contains these materials in the plate chamber for prolonged periods of time.
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Page 9: Chapter 1: Introduction
Chapter 1: Introduction The SpectraMax® M3 Multi-Mode Microplate Reader, SpectraMax® M4 Multi-Mode Microplate Reader, SpectraMax® M5 Multi-Mode Microplate Reader, and SpectraMax® M5e Multi-Mode Microplate Reader are dual monochromator, multi-detection instruments with a triple-mode cuvette port and a 6-well to 384-well plate read capability. The flexibility and high sensitivity makes these instruments appropriate for applications in the fields of biochemistry, cell biology, immunology, molecular biology, and microbiology. -
Page 10: Computer Integration
SpectraMax M5e) Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SoftMax Pro Software provides integrated instrument control, data display, and statistical data analysis. -
Page 11: Supported Plates And Cuvettes
Endpoint, Kinetic, and Spectrum read types are available for the Absorbance and Fluorescence read modes with cuvettes. Note: You can use a 12 x 75 mm test tube with a test tube cover. You can order the test tube cover from Molecular Devices. See Accessories on page 0112-0115 G... -
Page 12: Shake
SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Shake The software enables you to define settings to mix of the contents of the wells by shaking the plate linearly along the long axis at preset intervals. For the Endpoint read type, you can shake the plate for 0 to 999 seconds before the read at each wavelength you set. -
Page 13: Chapter 2: Setting Up The Instrument
Chapter 2: Setting Up the Instrument Before you unpack and set up the instrument, prepare a dry, flat work area that is away from direct sunlight, dust, drafts, vibration, and moisture. The work area must have sufficient space for the instrument, host computer, and required cables. To provide access for disconnecting power from the instrument, maintain a 20 cm to 30 cm (7.9 in. -
Page 14: Connecting Instrument Cables
SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9.8 foot) 4400-0002 Power cord, 1 meter (3.3 foot) See... -
Page 15: Chapter 3: Getting Started
Chapter 3: Getting Started Now that you have installed the SoftMax Pro Software software on the computer, removed the tape from the cuvette port, and connected the cables, it is time to get started. Turn on the instrument power switch located on the rear of the instrument. Wait for the instrument to complete its diagnostic check and the plate drawer opens. -
Page 16: Control Panel
SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Control Panel The instrument control panel works in conjunction with the SoftMax Pro Software to open the plate drawer, adjust the temperature, and to set the wavelengths for cuvette reads. See the SoftMax Pro Data Acquisition and Analysis Software User Guide. - Page 17 Chapter 3: Getting Started Temp The Temp p and q buttons enable you to enter the set point at which to regulate the temperature inside the cuvette chamber and the plate chamber. The temperature for the instrument chambers is isothermal at ambient temperature as well as at elevated temperatures.
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Page 18: Cuvette Chamber
SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Lift up the lid over the chamber to insert or remove a cuvette. The chamber contains springs that position the cuvette in the proper alignment for a read. -
Page 19: Plate Drawer
Chapter 3: Getting Started Plate Drawer The plate drawer is located on the right side of the instrument and slides in and out of the plate chamber. An internal latch positions the plate on the drawer as it closes. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. -
Page 20: Plate Adapter
SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer.
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