Molecular Devices SpectraMax M2 User Manual page 51

7. Specifications
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
46
Photometric stabilization
Photometric drift
Optical alignment
Average lamp lifetime
Photodetectors
Endpoint baseline noise (cuvette)
Endpoint kinetic noise (cuvette)
FLUORESCENCE PHOTOMETRIC PERFORMANCE
Detection limit (top read, microplate,
SpectraMax M2 and SpectraMax M2
Detection limit (bottom read, micro-
plate, SpectraMax M2
Detection limit (cuvette)
Excitation wavelength range
Emission wavelength range
Scanning provided over full range
Number of excitation/emission
pairs per plate
Bandwidth (excitation emission)
Dynamic range
System validation
Average lamp lifetime
Instantaneous
None—continuous referencing of
monochromatic input
Calibration Automatic before first kinetic read and before
every endpoint reading
None required
Light source Xenon flash lamp (50 Watts)
1 billion flashes
Silicon photodiode
±0.003 OD @190, 405, 850 nm
±0.003 OD @190, 405, 850 nm
≥ 0.2 mOD/min and ≤ 0.2 mOD/min
3.0 fmol/well in 200 µL FITC 96 wells (15 pM)
e
)
3.0 fmol/well in 75 µL FITC 384 wells (40 pM)
5.0 fmol/well FITC 200 µL in 96 wells (25 pM)
e
only)
5.0 fmol/well FITC 75 µL in 384 wells (67 pM)
15 pM fluorescein
250–850 nm
360–850 nm (SpectraMax M2)
250–850 nm (SpectraMax M2
in 1-nm increments
4
9/9 nm
6
10 in 96-well black plates: auto gain circuitry
Self-calibrating with built-in fluorescence cali-
brators
Light source Xenon flash lamp (1 joule/flash)
2 years normal operation
Detector Photomultiplier (R3896)
e
)