System Diagrams And Dimensions - Molecular Devices SpectraMax M2 User Manual

A. Appendix
Photomultiplier tube (PMT)
A vacuum tube that detects light especially from dim sources through the use of photoe-
mission and successive instances of secondary emission to produce enough electrons to
generate a useful current.
Read Mode
The type of reading performed: absorbance, fluorescence or luminescence.
Read Type
The method used to read the microplate: endpoint, kinetic, spectrum, or well scan.
Readings Per Well
The number of times (user-definable) that readings are taken on a well in fluorescence
mode or the amount of time that data is collected using the luminescence read type.
SoftMax Pro
An integrated software program from Molecular Devices Corporation that is used to con-
trol and collect data from all Molecular Devices plate readers.
Stokes Shift
The difference between the wavelengths of the excitation and emission peaks.
Time-Resolved Fluorescence
Most fluorescence substances are not suitable for this type of reading. However, the fluo-
rescence emitted by lanthanide dyes is delayed long enough to measure fluorescence after
the lamp is turned off. Time-resolved fluorescence is used to reduce the amount of back-
ground noise that interferes with fluorescence. The excitation lamp flashes and, after it is
off, the delayed emission is collected for a set period of time before the lamp is flashed
again.
A.9.

SYSTEM DIAGRAMS AND DIMENSIONS

Dimensions are shown in inches (millimeters).
Figure A.2: Front view of SpectraMax M2.
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
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