Description; Introduction - Molecular Devices SpectraMax M2 User Manual

1. Description

1.1.

INTRODUCTION

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The SpectraMax M2 and SpectraMax M2 Multi-detection Readers are monochromator-
based microplate readers that have 6-well to 384-well microplate reading capability as well
as a built-in absorbance and fluorescence cuvette port. The spectrophotometric
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performance of the SpectraMax M2 and SpectraMax M2 is similar to the SpectraMax
Plus, a dedicated absorbance plate reader. The fluorometric performance of the
SpectraMax M2 is similar to that of the Gemini XPS, a dedicated top-read fluorescence
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microplate reader. The fluorometric performance of the SpectraMax M2 is similar, but
slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence
microplate reader.
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SpectraMax M2 and SpectraMax M2 readers can acquire absorbance as well as
fluorescence data for samples by issuing a single read command in SoftMax Pro. Dual
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monochromators allow selection of any absorbance wavelength between 200 nm and
1000 nm, any excitation wavelength between 250 nm and 850 nm, and any emission
wavelength between 360 nm and 850 nm (SpectraMax M2) or 250 n and 850 nm
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(SpectraMax M2 ) for readings of both microplates and cuvettes.
1.1.1. APPLICATIONS
Endpoint, kinetic, spectrum, and multi-point well-scanning applications combining
absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint,
kinetic, and spectrum applications in absorbance and fluorescence using cuvettes, can be
run with little to no optimization.
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The extreme flexibility and high sensitivity of the SpectraMax M2 and SpectraMax M2
make them appropriate for applications within the fields of biochemistry, cell biology,
immunology, molecular biology, and microbiology.
Typical applications include ELISA, nucleic acid, protein, enzymatic type homogeneous
and heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration.
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The SpectraMax M2 and SpectraMax M2 also have two secondary modes that can be
used for limited development of glow luminescence or time-resolved fluorescence assays.
The performance of these two modes is not comparable to dedicated luminescence or
time-resolved fluorescence instruments, or multimode readers such as the
SpectraMax M5, Analyst HT or Analyst GT.
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator's Manual — 0112-0115 Rev. D
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