Fluorescence; Time-Resolved Fluorescence - Molecular Devices SpectraMax M2 User Manual

fluorochrome manufacturers are generally in methanol and do not reflect actual values
due to changes in pH, salt content, etc.
A.5.

FLUORESCENCE

Fluorophore
HPPA
4-MeU, NADH, NADPH
Biotinidinase
Green Fluorescent Protein
Attophos /Attofluor
Ethidium Homodmer (DNA)
TRITC, Ethidium Bromide
Texas Red
TAMRA
Tryptophan
La Jolla Blue
A.6.

TIME-RESOLVED FLUORESCENCE

Fluorophore
Eu-Chelate
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
Excitation Wavelength (nm)
PKU
FITC
Excitation Wavelength (nm)
360
Emission Wavelength (nm)
320
355
355
390
390
444
485
530
544
584
547
280
695
Emission Wavelength (nm)
610
A.5. Fluorescence
405
460
544
485
510
555
538
620
590
612
580
340
705
55