Molecular Devices SpectraMax M2 User Manual page 37

4. Operation
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
32
Figure 4.2 shows the effects of different cutoff filters on a scan of fluorescein where
excitation was fixed at 485 nm and emission was scanned from 490 nm to 560 nm (buffer
blanks are not shown in this plot). Table 4.1 (following) lists default settings for the
emission cutoff filters. For spectrum mode, the default is "manual" (no automatic cutoff ).
Table 4.1: Emission cutoff filter default settings.
Automatic Cutoff Selection
#
Wavelength (nm)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Endpoint and Kinetic Modes
Emission Wavelength (nm)
None
420
435
455
475
495
515
530
550
570
590
610
630
665
695
< 415
415–434
435–454
455–474
475–494
495–514
515–529
530–549
550–569
570–589
590–609
610–629
630–664
665–694
695–850