Read The Cuvette; Read The Microplate - Molecular Devices SpectraMax M2 User Manual

4.3.3. SELECT THE WAVELENGTH
For a cuvette reading, press the up or down arrow keys above and below the λ to
increment or decrement the wavelength setting in 1 nm increments.
4.4.

READ THE CUVETTE

Insert the cuvette into the chamber, making sure that the clear sides are to the left and
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right (facing the instrument). Do not touch the clear surfaces of the cuvette.
Make sure the cuvette is completely seated in the chamber and close the cuvette door.
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If the cuvette contains a blank (typically this solvent contains everything that the sam-
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ples contain except for analyte), press the
from the cuvette. The instrument automatically calibrate in less than two seconds,
closes the microplate drawer (if it is open), and reads the cuvette according to the
selected instrument settings.
If the cuvette contains a sample, touch the
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reading from the cuvette.
When the reading is complete, remove the cuvette.
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4.5.

READ THE MICROPLATE

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BIOHAZARD:
drawer. If the microplate has fluid on the underside, dry it using a paper towel (or
equivalent) before placing it in the drawer.
Insert the filled microplate into the drawer, matching well A1 with position A1 in the
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drawer. Make sure the microplate is flat against the drawer bottom (for 6-, 12-, 24-, or
48-well microplates) or against the adapter (if using top read for 96- or 386-well plates,
refer to the section "Installing the Drawer Adapter" in the chapter "Installation" in this
guide for more information).
You must have SoftMax Pro software running on a computer connected to the instru-
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ment. Press the READ button in SoftMax Pro to start the plate read.
When reading is complete, the drawer of the instrument opens, allowing you to remove
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the microplate. If the incubator is on, the drawer closes again after approximately 10
seconds.
If you return to the SpectraMax and find the drawer closed after a reading has finished,
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press the
For more information about plate reading, please consult the SoftMax Pro User Guide.
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. D
The underside of the microplate must be dry prior to placing it in the
key. When the drawer opens, you can remove the microplate.
DRAWER
4.4. Read the Cuvette
key to acquire the reference reading
REF
key to acquire the sample
READ CUVETTE
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