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TPH (Total Petroleum Hydrocarbons)
Using the 1-cm MicroCuvette Rack
Figure 2
The 1-cm MicroCuvette Rack
Interpreting and Reporting Results
TPH (Total Petroleum Hydrocarbons)
Page 8 of 12
This rack (see Figure 2) has been designed specifically to aid in achieving precise
and accurate results when using the immunoassay technique to analyze several
samples at the same time.
Loading the Rack — The cuvette rack is designed so that it may be inverted
with the cuvettes in place. Identify each cuvette with a sample or calibrator
number and place all the cuvettes in the rack before beginning the procedure. Fit
the cuvettes snugly into the rack, but do not force them or they may be difficult
to remove and their contents may spill. The cuvettes should remain in place
when the rack is inverted and tapped lightly.
Mixing — Set the rack on a hard, flat surface that is at least twice the length of
the rack. Hold the rack by one end and vigorously slide it back and forth along
its long axis for 30 seconds. The rack should move through a distance equal to its
own length in each direction.
There is an inverse relationship between the concentration of TPH and the
reading. In other words, the higher the reading, the lower the concentration
of TPH.
If the sample reading is...
...less than calibrator reading
...greater than calibrator reading
Example
Readings:
TPH Calibrator #1:
0.480 Abs
TPH Calibrator #2:
0.360 Abs
Sample #1:
0.200 Abs
the sample TPH Concentration is...
...greater than the calibrator concentration
...less than the calibrator concentration
TPHSoil_None_Other_IMM_Eng_Ody.fm
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