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13.
Wash each cuvette
forcefully and thoroughly
four times with deionized
water. Empty the rinse
water into the waste
container.
Note: Ensure most of the water
is drained from the cuvettes by
turning the cuvettes upside
down and tapping them lightly
on a paper towel.
Color Development
Note: Timing is critical; follow instructions carefully
1.
With the cuvettes still
held snugly in the rack,
pipet 0.5 mL of Color
Developing Solution into
each Antibody Cuvette.
Note: Use a new pipette tip for
each cuvette.
TPHSoil_None_Other_IMM_Eng_Ody.fm
TPH (Total Petroleum Hydrocarbons)
HRS MIN SEC
HRS MIN SEC
2.
Touch the timer icon.
Enter 10 minutes and
touch
OK.
A reaction period will
begin. Mix following the
instructions in Using the 1-
cm MicroCuvette Rack.
HRS MIN SEC
HRS MIN SEC
3.
After 5 minutes, mix
the contents of the rack a
second time for a period
of 30 seconds using the
same technique.
Note: Solutions will turn blue in
some or all of the cuvettes.
TPH (Total Petroleum Hydrocarbons)
4.
At the end of the
10-minute reaction
period, pipette 0.5 mL of
Stop Solution into each
cuvette in the same order
as the Color Developing
Solution was added in
step 1.
Slide the rack for 20
seconds using the
technique described in
Using the 1-cm
MicroCuvette Rack.
Note: Blue solutions will turn
yellow with the addition of the
Stop Solution.
Note: The same pipette tip can
be used repeatedly for this
step.
Page 5 of 12
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