Hach DR2400 Manual page 101

Sample Collection and Storage
Summary of Method
Required Reagents
Description
*
Reagent Set, Atrazine ........................................................................................................ 20 cuvettes ........27627-00
Required Apparatus
Adapter, 1-cm square cell .................................................................................................. each....................59459-00
Caps, flip spout ................................................................................................................... 2/pkg.................25818-02
Cell holder, 1-cm square .................................................................................................... each....................59065-00
Marker, laboratory.............................................................................................................. each....................20920-00
Rack, for 1-cm Micro Cuvettes.......................................................................................... each....................48799-00
Wipes, disposable ............................................................................................................... box .....................20970-00
®
TenSette , Pipet, 0.1–1.0 mL.............................................................................................. each....................19700-01
Tips, for pipettor 19700-01................................................................................................. 1000/pkg...........21856-28
Optional Reagents
Reagent Set, Atrazine ......................................................................................................... 100 cuvettes ......27627-10
* Immunoassay components are manufactured for Hach Company by Beacon Analytical Systems, Inc.
ATZ_None_Other_IMM_Eng_Ody.fm
Collect samples in a clean glass bottle. Do not pre-rinse the bottle with the
sample. If the sample cannot be analyzed immediately, store the sample at 4 °C.
Samples may be kept for as long as 14 days. Warm the samples to room
temperature before analysis.
Hach immunoassay tests use antigen/antibody reactions to test for specific
organic compounds in water and soil. Atrazine-specific antibodies, attached to
the walls of plastic cuvettes, selectively bind and remove Atrazine from complex
sample matrices. A prepared sample and a reagent containing enzyme-conjugate
molecules (analyte molecules attached to molecules of an enzyme) are added to
the Antibody Cuvettes. During incubation, enzyme-conjugate molecules and
Atrazine compete for binding sites on the antibodies. Samples with higher levels
of analyte will have more antibody sites occupied by Atrazine and fewer
antibody sites occupied by the enzyme-conjugate molecules.
After incubation, the sample and unbound enzyme conjugate are washed from
the cuvette and a color-development reagent is added. The enzyme in the
conjugate catalyzes the development of color. Therefore, there is an inverse
relationship between color intensity and the amount of Atrazine in the sample.
The resulting color is then compared with a calibrator to determine whether the
Atrazine concentration in the sample is greater or less than the threshold levels.
Test results are measured at 450 nm.
Atrazine
Unit Cat. No.
Atrazine
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