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DR/2400
Method 8178
Scope and Application:
* Adapted from Standard Methods for the Examination of Water and Wastewater
Tips and Techniques
• For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized
water in place of the sample. Subtract the reagent blank value from the final results or perform a reagent blank adjust
Section 2.6.2 Reagent Blank on page 20.
• Substitute the contents of one Amino Acid Reagent Powder Pillow (Cat. No. 804-99) for 1 mL of amino acid reagent solution in
step 4, if desired.
Hach Programs
1.
Touch
.
Hach Programs
Select program
.
485 P React. Amino
Touch
.
Start
PhosphorusReac_None_Other_AMI_Eng_Ody.fm
For water, wastewater, and seawater
2.
Fill a 25-mL mixing
cylinder with 25 mL of
sample.
Phosphorus, Reactive
(Orthophosphate)
(0.23 to 30.00 mg/L PO
3.
Add 1 mL of
Molybdate Reagent using
a 1-mL calibrated
dropper.
Phosphorus, Reactive (Orthophosphate)
Amino Acid Method*
3–
4
.
See
Method 8178
4.
Add 1 mL of Amino
Acid Reagent Solution.
Stopper and invert
several times to mix (this
is the prepared sample).
A blue color will form if
phosphate is present.
Page 1 of 6
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