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TPH (Total Petroleum Hydrocarbons)
5.
If testing soil: Pipet
0.5 mL of Diluent Solution
into each sample cuvette.
If testing water: Pipet
0.5 mL of each water
sample into the
appropriate cuvette.
Note: Use a new pipette tip for
each water sample.
HRS MIN SEC
HRS MIN SEC
9.
Touch the timer icon.
Enter 10 minutes and
press
OK.
A 10-minute reaction time
will begin. Proceed
immediately to the next
step.
TPH (Total Petroleum Hydrocarbons)
Page 4 of 12
6.
Have the necessary
apparatus at hand for the
next four steps as they
must be done without
delay.
®
Use a Wiretrol
pipet to
transfer 50 µL of each
calibrator to be used into
the calibrator cuvettes.
Mix the contents of the
cuvettes after each
addition.
Note: Use a separate capillary
tube for each solution.
10.
Mix the contents of
the cuvettes for
30 seconds using the
technique described in
Using the 1-cm
MicroCuvette Rack.
7.
If testing soil: Use a
Wiretrol pipet to transfer
50 µL of the filtered
extract from step 8 of the
Soil Extraction Procedure
into the appropriately
labeled cuvette.
Note: Use a separate capillary
tube for each solution.
Mix the contents of the
cuvettes after the addition
of each sample.
If testing water: Use a
Wiretrol pipet to transfer
50 µL of methanol into
each sample cuvette
HRS MIN SEC
HRS MIN SEC
11.
After 5 minutes, mix
the contents of the rack a
second time for a period
of 30 seconds using the
same technique.
8.
Immediately pipet
0.5 mL of TPH Enzyme
Conjugate into each
calibrator and
sample cuvette.
Note: The same pipette tip can
be used repeatedly for this
step.
12.
At the end of the
10-minute period, discard
the contents of all the
cuvettes into an
appropriate waste
container.
TPHSoil_None_Other_IMM_Eng_Ody.fm
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