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Summary of Contents for Beckman Coulter Allegra X-5
- Page 1 Instructions For Use Allegra X-5 Centrifuge Rx Only in the U.S.A. PN B29071AE April 2017 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 PN B29071AE (April 2017) ©2017 Beckman Coulter, Inc. All rights reserved Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision Status
Revision Status This document applies to the latest software listed and higher versions. When a subsequent software version affects the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com... - Page 4 Revision Status PN B29071AE...
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Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to... -
Page 6: Safety During Installation And/Or Maintenance
Safety Notice Safety During Installation and/or Maintenance Safety During Installation and/or Maintenance WARNING Risk of personal injury or equipment damage. The centrifuge weighs 77 kg/169 lb. DO NOT attempt to lift or move it without assistance from a lifting device and/or another person. -
Page 7: Safety Against Risk Of Fire
Safety Notice Safety Against Risk of Fire Safety Against Risk of Fire WARNING Risk of personal injury or equipment damage. This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) or materials which could react chemically with sufficient vigor to cause a HAZARD, nor handle or store them within the 30 cm (1ft) clearance envelope surrounding the centrifuge. - Page 8 • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. • Do not centrifuge materials that could result in a hazardous chemical reaction. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter. viii PN B29071AE...
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Page 9: Table Of Contents
Contents Revision Status, iii Safety Notice, v Alerts for Danger, Warning, Caution, Important, and Note, v Safety During Installation and/or Maintenance, vi Electrical Safety, vi Safety Against Risk of Fire, vii Mechanical Safety, vii Chemical and Biological Safety, vii Introduction, xv Intended Use, xv Certification, xv... - Page 10 Contents Centrifuge Function, 1-2 Safety Features, 1-2 Chassis, 1-3 Housing, 1-3 Door, 1-3 Rotor Chamber, 1-3 Drive, 1-4 Controls and Indicators, 1-4 Power Switch, 1-4 Control Panel, 1-5 System Keys, 1-5 Program Keys, 1-6 Digital Displays, 1-7 Name Rating Plate, 1-8 Specifications, 1-8 Rotor, 1-9 CHAPTER 2:...
- Page 11 Installation, A-1 Introduction, A-1 Installing the Instrument, A-1 Transportation Safety Devices, A-2 Transport Safety Device, A-2 Electrical Requirements, A-3 Test Run, A-5 APPENDIX B: Table of Hazardous Substance’s Name and Concentration, B-1 , B-1 Beckman Coulter, Inc. Allegra X-5 Centrifuge Warranty...
- Page 12 Illustrations Illustrations Front View, 1-1 Back View, 1-2 Interior View of the Rotor Chamber, 1-4 The Power Switch, 1-5 The Control Panel, 1-5 Digital Displays, 1-7 Tapered Sleeve Position, 2-2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers, 2-4 Default Parameters, 2-5 Location of the emergency door release.
- Page 13 Tables Tables Acceleration/Deceleration Times (in minutes:seconds), 2-7 Error Message Chart, 3-2 Troubleshooting Chart, 3-3 xiii...
- Page 14 Tables...
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Page 15: Introduction
Intended Use For In Vitro Diagnostic Use. This Allegra X-5 centrifuge is intended for the separation of components through the use of relative centrifugal force. It is designed to separate human samples, including blood and other body fluids, for processing, analysis, and in vitro diagnostic testing, as well as non-human body samples and chemicals, including industrial and environmental samples. -
Page 16: Conventions
Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user. -
Page 17: Caution Symbol
Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative. Protective Ground This symbol is used to indicate a protective ground. This instrument must be properly grounded. -
Page 18: Risk Of Fire Warning
For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device. -
Page 19: China Rohs Caution Label
Introduction Symbols and Labels China RoHS Caution Label This label and materials declaration table (APPENDIX B, Table of Hazardous Substance’s Name and Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. This label indicates that this electronic information product contains certain toxic or hazardous elements, and can be used safely during its environmental protection use period. -
Page 20: Consult Ifu Label
Introduction Symbols and Labels Consult IFU Label This label indicates that the Instructions for Use should be referred to for more information. IVD Label This label indicates an In Vitro diagnostic medical device. TUV-NRTL Label This label indicates recognition by a Nationally Recognized Test Laboratory (NRTL) that the instrument has met the relevant product safety standards. -
Page 21: Description
CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-5 Centrifuge. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). -
Page 22: Centrifuge Function
CHAPTER 2, Operation.) Safety Features The Allegra X-5 Centrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Instrument safety features include: • An electromechanical door-locking mechanism prevents operator contact with spinning rotors. -
Page 23: Chassis
Description Chassis • An overspeed system continuously monitors the rotor during centrifugation. The system includes a magnetic sensor on the drive motor. Throughout the run, checks are made to ensure that the rotor does not exceed set speed. • An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. -
Page 24: Drive
Description Controls and Indicators Figure 1.3 Interior View of the Rotor Chamber 1. Drive Shaft 3. Boot 2. Tapered Sleeve 4. Gasket Drive The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie- down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. -
Page 25: Control Panel
Description Controls and Indicators Figure 1.4 The Power Switch 1. Power Switch NOTE The power must be turned on before the chamber door can be opened. Control Panel The control panel is mounted at an angle on the front of the centrifuge and includes system keys, programming keys, and digital displays (see Figure 1.5). -
Page 26: Program Keys
Description Controls and Indicators START Pressing the START key causes the centrifuge run to begin. This key can also be used to abort a deceleration process and restart the centrifuge. STOP The STOP key can be pressed to end a run. It operates in two modes, depending on how you press it: •... -
Page 27: Digital Displays
Description Controls and Indicators TIME The TIME key is used to select the run duration. When the TIME key is pressed, the last digit on the TIME display flashes, indicating that the time can be entered with the cursor keys. •... -
Page 28: Name Rating Plate
The name rating plate is affixed to the right side of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge. -
Page 29: Rotor
Description Rotor Maximum altitude 2000 m (6562 ft). Dimensions Width 49.6 cm (19.5 in.) Depth 63.4 cm (25.0 in.) Height, door closed 39.0 cm (15.3 in.) Height, door open 88.0 cm (34.6 in.) Weight 77 kg (169 lb) Clearances (sides) 30 cm (11.8 in.) Electrical requirements 120-V instrument 120 VAC, 12A, 60 Hz... - Page 30 Description Rotor 1-10 PN B29071AE...
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Page 31: Chapter 2: Operation
CHAPTER 2 Operation Introduction This section contains operating procedures for the centrifuge, using the Beckman Coulter rotor designed for use in this centrifuge. Refer to the rotor manual for instructions on preparing the rotor for centrifugation. NOTE If the centrifuge is used in a manner other than that specified in this manual, the safety and performance of this equipment could be impaired. -
Page 32: Preparation And Loading
Figure 2.1 Tapered Sleeve Position 1. Tapered Sleeve NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service representative. Contact CAUTION Risk of equipment damage. Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor is forced sideways or dropped onto it. - Page 33 Operation Run Procedure Install the rotor according to the instructions in the rotor manual. a. Lower the rotor straight down onto the drive shaft. b. Tighten the tie-down screw clockwise onto the drive shaft. c. Seat the filled buckets onto the yoke pins. •...
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Page 34: Examples Of Correctly And Incorrectly Loaded Buckets And Carriers
Operation Run Procedure Remove the rotor from the centrifuge if a long period between runs is anticipated. CAUTION If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down screw is tight before each run. Figure 2.2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers 1. -
Page 35: Entering Run Parameters
Operation Run Procedure Entering Run Parameters When the power is applied for the first time (no previous runs), default values are displayed (see Figure 2.3). After the initial use, the parameters of the most recent run are displayed when power is applied. -
Page 36: Setting Rcf
Operation Run Procedure Press the ▲ or ▼ cursor key until the required RPM is displayed. • The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is displayed during the run. • You can check the RCF during the run by pressing the key while the centrifuge is running. -
Page 37: Setting Acceleration Rate
Operation Run Procedure Setting Acceleration Rate ACCEL key is used to select acceleration rates that protect delicate gradients. When the ACCEL key is pressed, the ACC/DEC display flashes, indicating that one of the 10 preset acceleration rates can be entered with the cursor keys. The selected rate depends on the type of run you are performing. -
Page 38: Starting A Run
Operation Run Procedure DECEL Press the key. ACC/DEC display flashes, indicating that the selected number can be entered with the cursor keys. Press the ▲ or ▼ cursor key until the required number is displayed. Starting a Run The run can be started using the parameters in memory from a previous run, or using new or changed parameters that you enter using the procedure described above. -
Page 39: Pulse Function
Operation Run Procedure CAUTION Risk of personal injury or equipment damage. Do not lift or move the centrifuge while the rotor is spinning. Do not place items on the centrifuge during operation. Pulse Function PULSE The pulse function, accessed by pressing the key, is used for short-duration runs. -
Page 40: Stopping A Run
When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com). -
Page 41: Enabling Or Disabling The Audible Tones
Operation Enabling or Disabling the Audible Tones Enabling or Disabling the Audible Tones The audible tones (beeps) that sound at the end of each run and if an error occurs can be turned on and off by following the steps below (rotor must be at 0 RPM). STOP Beep Press the... - Page 42 Operation Summary of Run Procedures ENTER Check that all parameters are correct and the door is shut and latched, then press then START WARNING Risk of personal injury. Do not attempt to override the door interlock system while the rotor is spinning or if there is any sound or vibration coming from the drive. Always wait for the rotor to come to a complete stop before attempting to open the centrifuge door.
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Page 43: Chapter 3: Troubleshooting
NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before requesting service by Beckman Coulter. User Messages CAUTION If the message SEr appears on the display, do not press any keys while the message is displayed. - Page 44 Troubleshooting User Messages Table 3.1 Error Message Chart Error Number Error Type Recommended Action Note • Allow to slow down System Error All these errors stop the centrifuge or cause it to • Power off/on decelerate brakeless • Allow to slow down 10-19 Speedometer error •...
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Page 45: Other Possible Problems
Troubleshooting Other Possible Problems Other Possible Problems Possible malfunctions that may not be indicated by diagnostic messages are described in Table 3.2, along with probable causes and corrective actions required. Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. -
Page 46: Location Of The Emergency Door Release
Troubleshooting Emergency Door Release WARNING Risk of personal injury. Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Turn the power off and disconnect the instrument from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such maintenance to qualified service personnel. -
Page 47: Insertion Of Allen Wrench
Troubleshooting Emergency Door Release Insert the supplied 5mm T-handle Allen wrench (P/N B31161) wrench horizontally into the hole (Figure 3.2). The wrench will be guided through a funnel-shaped tube to the shaft of the door lock motor. Figure 3.2 Insertion of Allen wrench. Turn the Allen wrench counter-clockwise to unlock the door. - Page 48 Troubleshooting Emergency Door Release PN B29071AE...
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Page 49: Care And Maintenance
NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before requesting service by Beckman Coulter Field Service. WARNING Risk of personal injury. Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury. -
Page 50: Cleaning
Care and Maintenance Cleaning To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning. Cleaning Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces. -
Page 51: Tube Breakage
Risk of personal injury. Flammability hazard. Do not use Ethanol or other flammable liquids in or near operating centrifuges. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 52: Storage And Transport
Care and Maintenance Storage and Transport Storage and Transport Storage Before storing a centrifuge for an extended period, return it to its original shipping container to protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge installation) into the chamber, making sure the drive shaft is stabilized in the hole in the foam. -
Page 53: Returning A Centrifuge
Figure 4.1 Locking Screws (Transport Safety Device) 1. Locking Screws Returning a Centrifuge Before returning a centrifuge or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. Contact us to obtain the authorization form and for packaging and shipping instructions. -
Page 54: Supply List
Refer to the applicable rotor manual for materials and supplies needed for rotors. Replacement Parts NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com. Description Part Number Rotor tie-down screw 368245... -
Page 55: Appendix A: Installation
APPENDIX A Installation Introduction This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available. WARNING Risk of personal injury or equipment damage. This centrifuge weigh 77kg/169 lbs. DO NOT attempt to lift or move it without assistance from a lifting device and/or another person. -
Page 56: Transportation Safety Devices
Installation Transportation Safety Devices Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge and resist vibration. • Refer to CHAPTER 1, Specifications for weight. a. -
Page 57: Electrical Requirements
Installation Electrical Requirements Figure A.1 Locking Screws (Transport Safety Device) 1. Locking Screws Save the Transport Safety Device Screws for future transport of the centrifuge. Electrical Requirements See the CHAPTER 1, Specifications for centrifuge electrical requirements. Make sure the voltage and frequency imprinted on the name rating plate affixed to the right side of the centrifuge agree with the line voltage and frequency of the outlet used. -
Page 58: Dimensions Of The Centrifuge
Installation Electrical Requirements Figure A.2 Dimensions of the Centrifuge NOTE Additional clearance is required on the right side to allow to allow access to the power switch. WARNING Risk of personal injury. Improper electrical connection could produce an electric shock. To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. -
Page 59: Test Run
The centrifuge must be plugged in and the power switch turned to on position (I) before the door can be opened. Beckman Coulter recommends that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See... - Page 60 Installation Test Run PN B29071AE...
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Page 61: Table Of Hazardous Substance's
APPENDIX B Table of Hazardous Substance’s Name and Concentration This materials declaration table (the Table of Hazardous Susbtance’s Name and Concentration) and China RoHS Caution Label are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006”Marking for Control of Pollution Caused by Electronic Information Products” requirements. - Page 62 有毒有害物质名称及吉量的标识格式 Table of Hazardous Substance’s Name and Concentration 电子电气产品号码 EEP Part Number: B30585, B30588 产品名称 Product Name: Benchtop Centrifuge 产品型号Product Model Number: Allegra X-5 部件名称 有毒有害物质或元素 Component Name Hazardous Substances Name 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 Cr6+ PBDE 印刷电路板组件 Printed Circuit Board Sub Assemblies 电缆组件...
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Page 63: Beckman Coulter, Inc
(1) year after delivery of the Allegra X-5 Centrifuge (the product), to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use. - Page 64 Beckman Coulter, Inc. Allegra X-5 Centrifuge Warranty Warranty-2 PN B29071AE...
- Page 65 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 符号...
- Page 66 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 符号...
- Page 68 Related Documents SX4700 Swinging-Bucket Rotor Instructions for Use PN B30343 Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 Use and Care of Tubes and Bottles PN IN-192 Available in hard copy or electronic pdf by request. Available at www.beckmancoulter.com www.beckmancoulter.com...
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