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Summary of Contents for Beckman Coulter Allegra X-14 sERIES
- Page 1 Instructions For Use Allegra X-14 Series Centrifuges Rx Only in the U.S.A. PN B07493AD January 2018 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 PN B07493AD (January 2018) © 2018 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision History
Revision History For labeling updates, go to techdocs.beckmancoulter.com and download the latest version of the manual or system help for your instrument. Issue AD, 01/18 Changes were made to: ECREP address. Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page. - Page 4 Revision History PN B07493AD...
- Page 5 Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.
- Page 6 Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge.
- Page 7 Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter. PN B07493AD...
- Page 8 Safety Notice Chemical and Biological Safety viii PN B07493AD...
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Page 9: Table Of Contents
Contents Revision History, iii Safety Notice, v Introduction, xiii CHAPTER 1: Description, 1-1 Introduction, 1-1 Centrifuge Function and Safety Features, 1-1 Centrifuge Function, 1-1 Safety Features, 1-2 Centrifuge Chassis, 1-2 Housing, 1-2 Door, 1-3 Rotor Chamber, 1-3 Temperature Sensing and Control, 1-3 Drive, 1-3 Controls and... - Page 10 List, 4-4 Replacement Parts, 4-4 Supplies, 4-4 APPENDIX A: Installation, A-1 Introduction, A-1 Space and Location Requirements, A-1 Electrical Requirements, A-2 Securing the Centrifuge to the Bench, A-3 Test Run, A-4 Beckman Coulter, Inc. Allegra X-14 Series Centrifuge Warranty Related Documents...
- Page 11 Illustrations Illustrations Centrifuge, 1-2 Control Panel, 1-4 Door Latch Override, 3-6 Centrifuge Dimensions, A-2...
- Page 12 Tables Tables Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed, 2-6 Diagnostic Message Chart, 3-2 Troubleshooting Chart, 3-5...
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Page 13: Safety Notice
Scope of Manual This manual is designed to familiarize you with the Beckman Coulter Allegra X-14 series centrifuges, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the... - Page 14 Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.
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Page 15: Description
CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-14 Series centrifuges. The operating controls and indicators are also described; instructions for their use are CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). -
Page 16: Safety Features
Figure 1.1 The Centrifuge Safety Features The Allegra X-14 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Safety features include the following. • An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and locked. -
Page 17: Door
Description Controls and Indicators Door The aluminum and structural foam door is secured to the housing by solid hinges. A window in the center allows strobe viewing. When the door is closed, the locking system engages. The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED next to the key is lit. -
Page 18: Control Panel
Description Controls and Indicators Control Panel The control panel (see Figure 1.2) is mounted at an angle on the centrifuge front for easy visibility and access. It is used to enter run parameters via function keys and a keypad, and to display run parameters, program information, and user messages. -
Page 19: Run-Parameter Keys
Description Controls and Indicators Displays the name of the selected rotor. ROTOR When the ACCEL key is pressed, one of ten available acceleration rates can be entered. Displays the selected acceleration rate throughout the run. ACCEL When the DECEL key is pressed, one of eleven available deceleration rates can be entered. -
Page 20: Name Rating Plate
Unlocks and opens the door. (The instrument will not accept the unlock command if the rotor is spinning.) Name Rating Plate A name rating plate is affixed to the rear of the instrument. Always mention the serial number and model number when contacting Beckman Coulter regarding your centrifuge. PN B07493AD... -
Page 21: Specifications
Description Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Specification Description Speed • Set speed — 200 to 10,200 RPM in 10-RPM increments • Speed display — actual rotor speed in 10-RPM increments or in RCF (when selected) Time •... - Page 22 Description Specifications Specification Description Electrical requirements • 120-V, 60-Hz instrument — 108–132 VAC, 12 A, 60 Hz Electrical supply Class I Maximum heat dissipation into room 4318 Btu/hr (1.3 kW) under steady-state conditions Noise level 0.91 m (3 ft) in front of ...
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Page 23: Available Rotors
Description Available Rotors Available Rotors The following Beckman Coulter rotors can be used in the Allegra X-14 series centrifuges. The rotors are described in individual manuals that accompany each rotor. Number Max RCF Rotor of Tubes Rotor ( g) - Page 24 Description Available Rotors 1-10 PN B07493AD...
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Page 25: Operation
CHAPTER 2 Operation Introduction This section contains centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. -
Page 26: Programmed Run
Operation Summary of Run Procedures Press , then press again or use the arrow keys to select the rotor. ROTOR ROTOR Press , then use the keypad to enter the run speed. Press a second time to RPM/RCF RPM/RCF toggle between RPM and RCF modes. Press , then use the keypad to enter the run time (to 99 hrs, 59 minutes);... -
Page 27: Preparation
Operation Preparation Check that all parameters are correct and that the door is closed (door LED will be lit). Press , then press (within 5 seconds). ENTER/SAVE START Wait for the run to end, or end the run by pressing STOP When the rotor stops (a tone sounds and the LED lights), press... -
Page 28: Manual Operation
When using multiwell-plate carriers, enter the appropriate rotor, either SX4750 or SX4750a. The speed will be automatically limited to 4000 RPM. (The 4000 RPM limit is valid when running up to three stacked Beckman Coulter plates separated by cap strips. Reduce speed to 2700 RPM if running four stacked plates). -
Page 29: Entering Run Time
Operation Manual Operation Use the keypad to enter required speed. • Entered speed appears on the speed display. • (If the entered speed is out of the speed range of the installed rotor, an error message will appear and the input will be cleared. Enter an acceptable value from the range displayed.) Press the next run-parameter key or ENTER/SAVE... -
Page 30: Entering Run Temperature
Operation Manual Operation Entering Run Temperature Run temperature can be set from 2 to +40°C. If no value is entered, the centrifuge selects the last entered temperature. Press TEMP • The cursor and the temperature range (2 to 40°C) appear. Use the keypad or arrow keys to enter required temperature. -
Page 31: Starting A Run
Operation Manual Operation Table 2.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed Rate SX4750 SX4750 SX4750A SX4750a FX6100 (4300 RPM) (4000 RPM) (4300 RPM) 4000 RPM (10,200 RPM) Accel Decel Accel Decel Accel Decel Accel Decel Accel Decel 2:45... -
Page 32: Pulse Function
Operation Programmed Operation a. (Press to terminate a run for any reason.) STOP • The green light next to the key will flash while the rotor decelerates. STOP • A tone sounds when the rotor comes to a complete stop. —... -
Page 33: Creating A New Program
Operation Programmed Operation Creating a New Program Press , then use the keypad or arrow keys to select a program number that is not in PROGRAM use, or one that you want to overwrite, and press ENTER/SAVE • The program number flashes. Enter run parameters (rotor, speed, time, temperature, acceleration/deceleration rate settings). -
Page 34: Disabling/Enabling The Audible Tones
Operation Disabling/Enabling the Audible Tones After the rotor has stopped and the LED by the door key lights, press DOOR • The door opens. Remove the rotor (or containers). Disabling/Enabling the Audible Tones The audible tones (beeps) that sound when a run-parameter key is pressed, or at the end of each run, may be turned on and off by following the steps below (rotor must be at 0 RPM). -
Page 35: Chapter 3: Troubleshooting
Maintenance procedures are given in CHAPTER 4. For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com) for assistance. NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories, before requesting service by Beckman Coulter Field Service. -
Page 36: Diagnostic Message Chart,
If door still doesn’t open call manual pressed Beckman Coulter Field Service. (Procedures for retrieving your sample are at the end of this section.) If the door opens but the message stays on the display, press CE to clear. - Page 37 Rotors CHAPTER 1). Check that magnets are in place on rotor bottom. Press CE to clear the message. • Call Beckman Coulter Field Service. R3– The entered rotor Run shuts down Press CE to clear message. Enter the correct Rotor ID mismatch,...
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Page 38: Other Possible Problems
Service calibrated a. In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office or visit us at www.beckmancoulter.com. Other Possible Problems Operating problems that may not be indicated by diagnostic messages are described in Table 3.2,... -
Page 39: Retrieving Your Sample In Case Of Power Failure
Have a qualified service person measure line set speed voltage while the instrument is operating. Electrical failure Make sure the power cord is securely connected; call Beckman Coulter Field Service. Motor failure Call Beckman Coulter Field Service. Door will not open Rotor spinning Wait until the rotor stops. - Page 40 Troubleshooting Retrieving Your Sample in Case of Power Failure Make sure that the rotor is not spinning. • No sound or vibration comes from the centrifuge. WARNING Never attempt to override the door interlock system while the rotor is spinning. Locate the two joined release cords underneath the bottom center of the centrifuge (not visible from the centrifuge front).
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Page 41: Chapter 4: Care And Maintenance
Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com) for assistance. User messages and recommended actions are discussed in CHAPTER 3, Troubleshooting. -
Page 42: Cleaning
Care and Maintenance Instrument Care If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use. a. To defrost the system, set the temperature to 30°C for 20 minutes. (These are suggested settings which may be adjusted as appropriate for your laboratory conditions.) NOTE Before using any cleaning or decontamination methods except those recommended by the... -
Page 43: Decontamination
See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials. While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 44: Storage And Transport
Storage and Transport Storage To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. Temperature and humidity conditions for storage should meet the environmental... -
Page 45: Appendix A: Installation
APPENDIX A Installation Introduction Preinstallation requirements have been sent prior to shipment of your centrifuge. WARNING This centrifuge weighs 128 kg (283 lb). Do not attempt to lift or move it without assistance. Space and Location Requirements WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids. -
Page 46: Electrical Requirements
Installation Electrical Requirements Ambient temperatures during operation should not be lower than 10°C (50°F) or higher than 30°C (86°F). • Relative humidity should not exceed 75% (noncondensing). Figure A.1 Centrifuge Dimensions 96.5 38.0 34.3 13.5 62.2 24.5 76.2 30.0 Electrical Requirements 120-V, 60-Hz centrifuge 108–132 VAC, 12 A, 60 Hz To reduce the risk of electrical shock, this centrifuge comes with a 2.5-m (8-ft) three-wire electrical... -
Page 47: Securing The Centrifuge To The Bench
Securing the Centrifuge to the Bench Allegra X-14 series centrifuges are certified to meet the centrifuge safety standards. To meet these requirements, the centrifuge must be secured to the bench using the anti-rotation kit shipped with the instrument. -
Page 48: Test Run
Installation Test Run Test Run NOTE The centrifuge must be plugged in and the power switch turned to on position (I) before the door can be opened. We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. -
Page 49: Beckman Coulter, Inc
(1) year after delivery of an Allegra X-14 series centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use. - Page 50 Beckman Coulter, Inc. Allegra X-14 Series Centrifuge Warranty Warranty-2 PN B07493AD...
- Page 51 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 符号...
- Page 52 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 符号...
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Page 54: Related Documents
Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 Available in hard copy or electronic pdf by request. Available at www.beckmancoulter.com RPM-to-RCF Conversion Chart when Running Multiwell-plate Carriers in the SX4750A Rotor PN GX-TB-015 Available in hard copy or electronic pdf by request.
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