Beckman Coulter Allegra X-30 Series Instructions For Use Manual
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Beckman Coulter Allegra X-30 Series Instructions For Use Manual

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Instructions For Use
Allegra X-30 Series Centrifuges
Rx Only
PN B05529AE
September 2018
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

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  • Page 1 Instructions For Use Allegra X-30 Series Centrifuges Rx Only PN B05529AE September 2018 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 PN B05529AE (September 2018) ©2018 Beckman Coulter, Inc. All rights reserved Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

  • Page 3: Revision History

    Revision History For labeling updates, go to www.beckman.com and download the latest version of the manual or system help for your instrument. Issue AD, 11/2015 Changes were made to: Introduction, CE Mark. Issue AE, 09/2018 Changes were made to: CHAPTER 1, Available Rotors.
  • Page 4 Revision History PN B05529AE...

  • Page 5: Safety Notice

    Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as...

  • Page 6: Electrical Safety

    Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.

  • Page 7: Chemical And Biological Safety

    Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter. PN B05529AE...
  • Page 8 Safety Notice Chemical and Biological Safety viii PN B05529AE...

  • Page 9: Table Of Contents

    Contents Revision History, iii Safety Notice, v Alerts for Danger, Warning, Caution, Important, and Note, v Safety During Installation and/or Maintenance, v Electrical Safety, vi Safety Against Risk of Fire, vi Mechanical Safety, vi Chemical and Biological Safety, vii Introduction, xv Intended Use, xv Certification, xv...
  • Page 10 Contents Name Rating Plate, 1-8 Nonrefrigerated Model Specifications, 1-8 Refrigerated Model Specifications, 1-9 Available Rotors, 1-10 CHAPTER 2: Operation, 2-1 Introduction, 2-1 Procedure, 2-2 Preparation and Loading, 2-2 Entering Run Parameters, 2-6 Starting a Run, 2-10 Pulse Function, 2-11 Changing Parameters During a Run, 2-11 Stopping a Run, 2-12...
  • Page 11 Contents APPENDIX A: Installation, A-1 Introduction, A-1 Installing the Instrument, A-1 Transportation Safety Devices, A-2 Electrical Requirements, A-2 Test Run, A-4 Beckman Coulter, Inc. Allegra X-30 Series Centrifuge Warranty...
  • Page 12 Illustrations Illustrations Interior View of the Rotor Chamber, 1-3 The Power Switch, 1-4 System Panels, 1-4 System Keys, 1-5 Program Keys, 1-6 Digital Displays, 1-7 Tapered Sleeve Position, 2-3 Default Parameters, 2-6 Screw Locations, 3-4 Internal Access, 3-5 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges, A-3...
  • Page 13 Tables Tables Acceleration/Deceleration Times (in minutes:seconds), 2-9 Error Message Chart, 3-2 Troubleshooting Chart, 3-3 xiii...
  • Page 14 Tables...

  • Page 15: Introduction

    Scope of Manual This manual is designed to familiarize you with the Beckman Coulter Allegra X-30 Series centrifuges, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the Safety Notice section and all safety- related information, before operating the centrifuge or performing instrument maintenance.

  • Page 16: Conventions

    Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

  • Page 17: Ce Mark

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
  • Page 18 Introduction CE Mark xviii PN B05529AE...

  • Page 19: Description

    CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-30 Series centrifuges. The operating controls and indicators are also described; instructions for their use are CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175).

  • Page 20: Safety Features

    Description Chassis Safety Features Allegra X-30 Series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Instrument safety features include: • The door has an electromechanical door-locking mechanism to prevent operator contact with spinning rotors.

  • Page 21: Drive

    Description Temperature Sensing and Control (refrigerated models only) Figure 1.1 Interior View of the Rotor Chamber 1. Drive Shaft 4. Gasket 2. Tapered Sleeve 5. Rotor Detector 3. Boot 6. Thermistor (refrigerated models only) Drive The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie- down screw is used to attach the rotor to the drive shaft.

  • Page 22: Controls And Indicators

    Description Controls and Indicators Controls and Indicators Power Switch The power switch is located on the centrifuge right side panel (see Figure 1.2). This two-position rocker switch ( , on; , off) controls electrical power to the centrifuge. Figure 1.2 The Power Switch 1.

  • Page 23: System Keys

    Description Controls and Indicators System Keys The centrifuge operation is controlled through the system keys (see Figure 1.4). Each key (except key) has an LED in the upper left corner that lights to indicate that the key can be PULSE activated.

  • Page 24: Program Keys

    Description Controls and Indicators Figure 1.5 Program Keys ▲ ▼ The cursor keys are up and down arrow keys (▲ and ▼), which can be pressed to (cursor keys) increment values up or down when setting parameters. ENTER Parameter (speed, time, temperature, and acceleration or deceleration curve) changes made while a run is in progress must be verified by pressing the ENTER key.

  • Page 25: Digital Displays

    Description Controls and Indicators Digital Displays Digital displays indicate rotor speed, run time, rotor chamber temperature, and numbers that represent selected acceleration and deceleration profiles (see Figure 1.6). When the power is turned on, they show the operating parameters of the most recent run performed before the power was turned off.

  • Page 26: Name Rating Plate

    The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

  • Page 27: Refrigerated Model Specifications

    Description Refrigerated Model Specifications Noise level 0.91 m (3 ft) in front of £68 dBa instrument (approx.) Installation (overvoltage) category Pollution degree a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected. Refrigerated Model Specifications Only values with tolerances or limits are guaranteed data.

  • Page 28: Available Rotors

    Description Available Rotors Electrical supply Class I Maximum heat dissipation into room under 3311 Btu/h (0.97 kW) steady-state conditions Noise level 0.91 m (3 ft) in front of £68 dBa instrument (approx.) Installation (overvoltage) category Pollution degree a. Temperature range depends on rotor in use and speed (see applicable rotor manual). b.
  • Page 29 Description Available Rotors Rotor Profile Description Refrigerated Nonrefrigerated Rotor Part Capacity Number/ Max RCF (mL) Rotor Manual ( g) Number ( g) F1010 18,000 27,579 16,000 21,791 10  10 361221 Fixed Angle GS-TB-007 35° Angle = 76 mm C0650 (Conical) 10,000 10,304 9000...
  • Page 30 Description Available Rotors 1-12 PN B05529AE...

  • Page 31: Chapter 2: Operation

    Operation Introduction This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power turned off ( ) when the centrifuge is not in use.

  • Page 32: Run Procedure

    Operation Run Procedure WARNING Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Operator error or tube failure may generate aerosols. Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken.

  • Page 33: Tapered Sleeve Position

    1. Tapered Sleeve NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).
  • Page 34 Operation Run Procedure a. Lower the yoke straight down onto the drive shaft. b. Tighten the tie-down screw clockwise onto the drive shaft. c. Seat the filled buckets onto the yoke pins. • Be sure to fill all positions on the yoke with buckets. For a fixed angle rotor, the steps include the following: PN B05529AE...
  • Page 35 Operation Run Procedure a. Lower the rotor straight down onto the drive shaft. b. Tighten the tie-down screw clockwise onto the drive shaft. c. Attach the lid and tighten it. Some rotors have a knob and others have a bolt for the T-handle wrench.

  • Page 36: Entering Run Parameters

    Operation Run Procedure CAUTION Always run any type of rotor with a balanced load. Close the centrifuge door and push firmly down on both sides of the door front until you hear a clicking (latching) sound. Remove the rotor from the centrifuge if a long period between runs is anticipated. CAUTION If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down screw is tight before each run.
  • Page 37 Operation Run Procedure Selecting a Rotor Number Each rotor that can be run in the centrifuge has a rotor number; the number is engraved on the rotor (and on the lid of fixed angle rotors). The centrifuge internal memory contains a list of all rotors that can be run in the centrifuge, together with acceptable run parameters for each rotor.
  • Page 38 Operation Run Procedure Setting RCF Press the key. • The last digit on the display ( ) flashes, indicating that you can enter the RCF. SPEED Press the ▲ or ▼ cursor key until the required RCF is displayed. • The corresponding RPM is automatically calculated and the centrifuge runs at the calculated speed.
  • Page 39 Operation Run Procedure display flashes, indicating that the temperature can be entered with the TEMP°C cursor keys. Press the ▲ or ▼ cursor key until the required run temperature is displayed. NOTE For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

  • Page 40: Starting A Run

    Operation Run Procedure a. Times are approximate; actual times vary depending on the rotor in use, the rotor load, run speed, and voltage fluctuations. b. Maximum speed for the S2096 microtiter rotor is 3000 rpm; acceleration and deceleration times decrease accordingly. c.

  • Page 41: Pulse Function

    Operation Run Procedure • A blinking LED at the bottom of the display indicates that the run is in progress. TIME — This display also shows the time remaining in the run (or  and elapsed time for continuous operation). WARNING Do not attempt to override the door interlock system while the rotor is spinning.

  • Page 42: Stopping A Run

    When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

  • Page 43: Disabling The Audible Tones

    Operation Disabling the Audible Tones After completing a run, unload the rotor following the instructions in the applicable rotor manual. CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.
  • Page 44 Operation Summary of Run Procedures Close the centrifuge door and push firmly down on it until you hear the latch engage. Enter run parameters: a. Select a rotor number — , ▲ or ▼, ROTOR ENTER b. Set run speed — , ▲...

  • Page 45: Chapter 3: Troubleshooting

    If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, gather as much information about the situation as you can: •...
  • Page 46 If the recommended action does not correct the problem, call Beckman Coulter Field Service. b. In the United States, call 1-800-742-2345; outside the U.S., contact your local Beckman Coulter office or visit us at www.beckmancoulter.com. PN B05529AE...

  • Page 47: Other Possible Problems

    Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service. Table 3.2 Troubleshooting Chart...

  • Page 48: Emergency Access

    Troubleshooting Emergency Access Emergency Access If the facility power fails only momentarily, the centrifuge resumes operation when power is restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have to restart the run when the power is restored. In the event of an extended power failure, you may have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

  • Page 49: Internal Access

    Troubleshooting Emergency Access Set the covers aside. Insert a Phillips-head screwdriver through one of the exposed holes in the centrifuge front panel and loosen, but do not remove, the screw. a. Repeat for the other screw. Move the centrifuge forward on the bench until the two screws on the bottom of the centrifuge are accessible (see Figure 3.1).
  • Page 50 Troubleshooting Emergency Access DANGER Never try to slow or stop the rotor by hand. After removing the rotor, replace the front panel, screws, and covers. PN B05529AE...

  • Page 51: Chapter 4: Care And Maintenance

    CHAPTER 4 Care and Maintenance Introduction For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are discussed in CHAPTER 3, Troubleshooting. Refer to the applicable rotor manual and to Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

  • Page 52: Cleaning

    Care and Maintenance Cleaning Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber icing (refrigerated models). a. If chamber icing occurs, defrost before use. To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning.

  • Page 53: Tube Breakage

    Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 54: Storage And Transport

    Returning a Centrifuge Before returning a centrifuge or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the form and for packaging and shipping instructions. To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity.
  • Page 55 Care and Maintenance Supply List Description Part Number Silicone vacuum grease (1 oz) 335148 Beckman Solution 555 (1 qt) 339555 PN B05529AE...
  • Page 56 Care and Maintenance Supply List PN B05529AE...

  • Page 57: Appendix A: Installation

    APPENDIX A Installation Introduction This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available. WARNING These centrifuges each weigh 48 kg/106 lb (non-refrigerated model) or 78 kg/172 lb (refrigerated model.) DO NOT attempt to lift or move one of them without assistance from a lifting device or another person.

  • Page 58: Transportation Safety Devices

    Installation Transportation Safety Devices a. Make sure that the centrifuge front feet are fully supported on the table. b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation. c. Check that there are 7.6-cm (3-in.) clearances at the sides of the centrifuge to ensure sufficient air circulation.

  • Page 59: Dimensions Of The Refrigerated And Nonrefrigerated Centrifuges

    Installation Electrical Requirements Figure A.1 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges NOTE Additional clearance is required on the right side to allow to allow access to the power switch. PN B05529AE...

  • Page 60: Test Run

    Installation Test Run WARNING To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.

  • Page 61: Beckman Coulter, Inc

    (1) year after delivery of the Allegra X-30 series centrifuge (the product), to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
  • Page 62 Beckman Coulter, Inc. Allegra X-30 Series Centrifuge Warranty Warranty-2 PN B05529AE...
  • Page 64 Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 Available in hard copy or electronic pdf by request. Available at www.beckman.com www.beckman.com © 2018 Beckman Coulter, Inc. All Rights Reserved...

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